Kaur Jasreen, Kelpsiene Egle, Gupta Govind, Dobryden Illia, Cedervall Tommy, Fadeel Bengt
Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet Nobels väg 13 171 77 Stockholm Sweden
NanoLund, Department of Biochemistry and Structural Biology, Lund University Lund Sweden.
Nanoscale Adv. 2023 May 30;5(13):3453-3462. doi: 10.1039/d3na00323j. eCollection 2023 Jun 27.
Micro- and nanoplastic pollution has emerged as a global environmental problem. Moreover, plastic particles are of increasing concern for human health. However, the detection of so-called nanoplastics in relevant biological compartments remains a challenge. Here we show that Raman confocal spectroscopy-microscopy can be deployed for the non-invasive detection of amine-functionalized and carboxy-functionalized polystyrene (PS) nanoparticles (NPs) in . The presence of PS NPs in the gastrointestinal (GI) tract of was confirmed by using transmission electron microscopy. Furthermore, we investigated the ability of NH-PS NPs and COOH-PS NPs to disrupt the epithelial barrier of the GI tract using the human colon adenocarcinoma cell line HT-29. To this end, the cells were differentiated for 21 days and then exposed to PS NPs followed by cytotoxicity assessment and transepithelial electrical resistance measurements. A minor disruption of barrier integrity was noted for COOH-PS NPs, but not for the NH-PS NPs, while no overt cytotoxicity was observed for both NPs. This study provides evidence of the feasibility of applying label-free approaches, , confocal Raman mapping, to study PS NPs in a biological system.
微塑料和纳米塑料污染已成为一个全球性的环境问题。此外，塑料颗粒对人类健康的影响日益受到关注。然而，在相关生物区室中检测所谓的纳米塑料仍然是一项挑战。在这里，我们展示了拉曼共聚焦光谱显微镜可用于非侵入性检测胺官能化和羧基官能化的聚苯乙烯（PS）纳米颗粒（NPs）。通过透射电子显微镜证实了PS NPs在[具体生物名称]胃肠道（GI）中的存在。此外，我们使用人结肠腺癌细胞系HT - 29研究了NH - PS NPs和COOH - PS NPs破坏胃肠道上皮屏障的能力。为此，将细胞分化21天，然后暴露于PS NPs，随后进行细胞毒性评估和跨上皮电阻测量。注意到COOH - PS NPs对屏障完整性有轻微破坏，但NH - PS NPs没有，而两种NPs均未观察到明显的细胞毒性。本研究提供了应用无标记方法，即共聚焦拉曼映射，在生物系统中研究PS NPs的可行性证据。
