Department of Physics, Faculty of Science, University of Hradec Králové, Rokitanského 62, Hradec Králové, 500 03, Czech Republic.
Department of Epidemiology, University of Defencein Brno, Třebešská, 1575, 500 02, Czech Republic.
J Biol Phys. 2023 Sep;49(3):383-392. doi: 10.1007/s10867-023-09638-y. Epub 2023 Jun 30.
In this study, scanning electron microscopy (SEM) was used to study the cell structure of SARS-CoV-2 infected cells. Our measurements revealed infection remodeling caused by infection, including the emergence of new specialized areas where viral morphogenesis occurs at the cell membrane. Intercellular extensions for viral cell surfing have also been observed. Our results expand knowledge of SARS-CoV-2 interactions with cells, its spread from cell to cell, and their size distribution. Our findings suggest that SEM is a useful microscopic method for intracellular ultrastructure analysis of cells exhibiting specific surface modifications that could also be applied to studying other important biological processes.
在这项研究中，扫描电子显微镜（SEM）被用于研究 SARS-CoV-2 感染细胞的细胞结构。我们的测量结果揭示了感染引起的细胞重构，包括在细胞膜上发生病毒形态发生的新的专门区域的出现。也观察到了用于病毒细胞冲浪的细胞间延伸。我们的结果扩展了对 SARS-CoV-2 与细胞相互作用、病毒在细胞间传播及其大小分布的认识。我们的发现表明，SEM 是一种用于分析表现出特定表面修饰的细胞的细胞内超微结构的有用显微镜方法，也可应用于研究其他重要的生物学过程。
