利用高分辨率扫描电子显微镜对 SARS-CoV-2 与宿主细胞的相互作用进行超微结构分析。
Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy.
机构信息
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Cidade Universitária. Av., Carlos Chagas Filho 373, Prédio CCS, Bloco C, subsolo, CEP: 21941902, Rio de Janeiro, RJ, Brazil.
Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Rio de Janeiro, Brazil.
出版信息
Sci Rep. 2020 Sep 30;10(1):16099. doi: 10.1038/s41598-020-73162-5.
SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.
SARS-CoV-2 是引发当前 COVID-19 大流行的病原体。在这里，我们使用高分辨率扫描电子显微镜研究了这种新型冠状病毒与 Vero 细胞的相互作用。在感染后 2 小时和 48 小时，观察了表面形态、感染细胞内部以及两种环境中病毒颗粒的分布。我们展示了病毒加工区域、空泡内容物的细节以及病毒与细胞表面的相互作用。还观察到了细胞间连接，病毒颗粒附着在这些延伸物上，表明 SARS-CoV-2 可以通过细胞间直接传播。
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