Liu Siying, Zheng Chuanjian, Hao Qun, Li Xin, Zhang Shaohui
Opt Lett. 2023 Jul 1;48(13):3559-3562. doi: 10.1364/OL.493167.
We propose a single-shot quantitative differential phase contrast method with polarization multiplexing illumination. In the illumination module of our system, a programmable LED array is divided into four quadrants and covered with polarizing films of four different polarization angles. We use a polarization camera with polarizers before the pixels in the imaging module. By matching the polarization angle between the polarizing films over the custom LED array and the polarizers in the camera, two sets of asymmetric illumination acquisition images can be calculated from a single-shot acquisition image. Combined with the phase transfer function, we can calculate the quantitative phase of the sample. We present the design, implementation, and experimental image data demonstrating the ability of our method to obtain quantitative phase images of a phase resolution target, as well as Hela cells.
我们提出了一种具有偏振复用照明的单次定量微分相衬方法。在我们系统的照明模块中，一个可编程LED阵列被分成四个象限，并覆盖有四个不同偏振角度的偏振膜。我们在成像模块的像素之前使用带有偏振器的偏振相机。通过匹配定制LED阵列上的偏振膜与相机中的偏振器之间的偏振角度，可以从单次采集图像中计算出两组不对称照明采集图像。结合相位传递函数，我们可以计算出样品的定量相位。我们展示了该方法的设计、实现和实验图像数据，证明了我们的方法能够获得相位分辨率目标以及Hela细胞的定量相位图像。
