从丝状真菌Hypoxylon fendleri 中分离到一种新型的丝状瘤病毒。
A novel mitovirus isolated from the filamentous fungus Hypoxylon fendleri.
机构信息
Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Engineering Research Center of Cellular Immunotherapy of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, China.
Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, 550025, China.
出版信息
Arch Virol. 2023 Jul 1;168(7):198. doi: 10.1007/s00705-023-05811-9.
Members of the fungal genus Hypoxylon of the family Xylariaceae are known to produce secondary metabolites with significant chemical diversity. There are more than 200 species in the genus, including the filamentous fungus Hypoxylon fendleri. To the best of our knowledge, there have been no reports of mycoviruses in H. fendleri. In this study, a novel mycovirus, designated "Hypoxylon fendleri mitovirus 1" (HfMV1), was isolated from this fungus. The genome of HfMV1 is 2850 nt in length with a G + C content of 36% and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). BLASTp analysis revealed that the RdRp domain of HfMV1 had 28.30-50.90% sequence identity to those of members of the genus Duamitovirus and had the highest identity (50.90%) to Fusarium graminearum mitovirus 2-2 (FgMV2-2). Phylogenetic analysis further indicated that HfMV1 is a member of the genus Duamitovirus of the family Mitoviridae. This is the first report of a mycovirus in H. fendleri.
真菌木霉属（Xylariaceae 科）的成员已知会产生具有显著化学多样性的次生代谢物。该属有超过 200 种物种，包括丝状真菌 Hypoxylon fendleri。据我们所知，在 H. fendleri 中尚未发现真菌病毒。在这项研究中，从这种真菌中分离到一种新型真菌病毒，命名为“Hypoxylon fendleri mitovirus 1”（HfMV1）。HfMV1 的基因组长 2850 个核苷酸，G+C 含量为 36%，包含一个编码 RNA 依赖性 RNA 聚合酶（RdRp）的大开放阅读框（ORF）。BLASTp 分析表明，HfMV1 的 RdRp 结构域与 Duamitovirus 属成员具有 28.30-50.90%的序列同一性，与 Fusarium graminearum mitovirus 2-2（FgMV2-2）的同一性最高（50.90%）。系统发育分析进一步表明，HfMV1 是 Mitoviridae 科 Duamitovirus 属的一员。这是 H. fendleri 中真菌病毒的首次报道。
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