Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People's Republic of China.
Arch Virol. 2021 Feb;166(2):633-637. doi: 10.1007/s00705-020-04886-y. Epub 2020 Nov 22.
Here, a novel mycovirus, Botryosphaeria dothidea mitovirus 1 (BdMV1), was isolated from a phytopathogenic fungus, Botryosphaeria dothidea, and its molecular characteristics were determined. BdMV1 has a genome of 2,667 nt that contains a single large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF encodes an RNA-dependent RNA polymerase (RdRp) of 727 amino acids with a molecular mass of 81.64 kDa. BLASTp analysis revealed that the RdRp domain of BdMV1 has 39.59% and 39.18% sequence identity to Plasmopara viticola associated mitovirus 43 and Setosphaeria turcica mitovirus 1, respectively. Phylogenetic analysis further suggested that BdMV1 is a new member of the genus Mitovirus within the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus in B. dothidea.
这里，我们从一种植物病原菌胶孢炭疽菌中分离到了一种新的真菌病毒，命名为胶孢炭疽菌呼肠孤病毒 1（BdMV1），并对其分子特征进行了测定。BdMV1 的基因组大小为 2667nt，采用真菌线粒体遗传密码，含有一个单一的大开放阅读框（ORF）。ORF 编码一个由 727 个氨基酸组成的 RNA 依赖性 RNA 聚合酶（RdRp），分子量为 81.64kDa。BLASTp 分析表明，BdMV1 的 RdRp 结构域与 Plasmopara viticola 相关呼肠孤病毒 43 和 Setosphaeria turcica 呼肠孤病毒 1 分别具有 39.59%和 39.18%的序列同一性。系统进化分析进一步表明，BdMV1 是呼肠孤病毒科呼肠孤病毒属的一个新成员。据我们所知，这是胶孢炭疽菌中呼肠孤病毒的首次报道。
