Hao Junfeng, Wang Zhijun, Jia Yushan, Sun Lin, Fu Zhihui, Zhao Muqier, Li Yuyu, Yuan Ning, Cong Baiming, Zhao Lixing, Ge Gentu
Key Laboratory of Forage Cultivation and the Processing and High Efficient Utilization of the Ministry of Agriculture, Key Laboratory of Grassland Resources of the Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, China.
Front Nutr. 2023 Jun 15;10:1065662. doi: 10.3389/fnut.2023.1065662. eCollection 2023.
In this study, the ultrasound-assisted extraction (UAE) conditions of flavonoids from L.cv. Mengzao (LIM) leaves was optimized, and the flavonoids content and their antioxidant potential in different parts were analyzed. The optimal extraction parameters to obtain the highest total flavonoids content (TFC) were a a ratio of liquid to solid of 24.76 mL/g, ultrasonic power of 411.43 W, ethanol concentration of 58.86% and an extraction time of 30 min, the average TFC of LIM leaves could reach 48.01 mg/g. For the yield of flavonoids, the UAE method had the best extraction capacity compared with solvent extraction and microwave-assisted extraction (MAE). In general, the TFC in different parts of LIM followed the order flower > leaf > stem > root, the flowering period is the most suitable harvesting period. From ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) quantification, the flower samples showed significantly higher six flavonoids and had the highest radical scavenging capacities compared to other samples. A high positive correlation was observed between the antioxidant activity and TFC, luteolin-7-O-glucoside and rutin were significantly ( < 0.05) correlated with all antioxidant evaluations. This study provides valuable information for the development and utilization of flavonoids in as ingredients in food, feed and nutritional health products.
本研究优化了超声辅助提取（UAE）蒙自龙眼（LIM）叶片中黄酮类化合物的条件，并分析了不同部位黄酮类化合物的含量及其抗氧化潜力。获得最高总黄酮含量（TFC）的最佳提取参数为液固比24.76 mL/g、超声功率411.43 W、乙醇浓度58.86%和提取时间30 min，LIM叶片的平均TFC可达48.01 mg/g。就黄酮类化合物的产量而言，与溶剂提取和微波辅助提取（MAE）相比，UAE法具有最佳的提取能力。总体而言，LIM不同部位的TFC遵循花＞叶＞茎＞根的顺序，花期是最适宜的采收期。通过超高效液相色谱-质谱联用（UPLC-MS）定量分析，花样品中六种黄酮类化合物含量显著更高，与其他样品相比具有最高的自由基清除能力。抗氧化活性与TFC之间存在高度正相关，木犀草素-7-O-葡萄糖苷和芦丁与所有抗氧化评价指标均呈显著（<0.05）相关。本研究为LIM中黄酮类化合物作为食品、饲料和营养保健品成分的开发利用提供了有价值的信息。
