欧洲越橘花青素通过抑制TNF-α/TGF-β/IFN-γ/NF-kB/半胱天冬酶-3信号通路对大鼠酒精性肝损伤的影响

Effect of Europinidin against Alcohol-Induced Liver Damage in Rats by Inhibiting the TNF-α/TGF-β/IFN-γ/NF-kB/Caspase-3 Signaling Pathway.

作者信息

Mahdi Wael A, AlGhamdi Shareefa A, Alghamdi Amira M, Imam Syed Sarim, Alshehri Sultan, Almaniea Mohammad A, Hajjar Baraa Mohammed, Al-Abbasi Fahad A, Sayyed Nadeem, Kazmi Imran

机构信息

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

出版信息

ACS Omega. 2023 Jun 12;8(25):22656-22664. doi: 10.1021/acsomega.3c01312. eCollection 2023 Jun 27.

BACKGROUND

The effect of europinidin on alcoholic liver damage in rats was examined in this research.

METHODS

A total of 24 Wistar rats were grouped in the same way into four groups: normal control (normal), ethanol control (EtOH), europinidin low dose (10 mg/kg), and europinidin higher dose (20 mg/kg). The test group rats were orally treated with europinidin-10 and europinidin-20 for 4 weeks, whereas 5 mL/kg distilled water was administered to control rats. In addition, 1 h after the last dose of the above-mentioned oral treatment, 5 mL/kg (i.p.) EtOH was injected to induce liver injury. After 5 h of EtOH treatment, samples of blood were withdrawn for biochemical estimations.

RESULTS

Administration of europinidin at both doses restored all of the estimated serum, i.e., liver function tests (ALT, AST, ALP), biochemical test (Creatinine, albumin, BUN, direct bilirubin, and LDH), lipid assessment (TC and TG), endogenous antioxidants (GSH-Px, SOD, and CAT), malondialdehyde (MDA), nitric oxide (NO), cytokines (TGF-β, TNF-α, IL-1β, IL-6, IFN-γ, and IL-12), caspase-3, and nuclear factor kappa B (NF-κB) associated with the EtOH group.

CONCLUSION

The results of the investigation showed that europinidin had favorable effects in rats given EtOH and may have hepatoprotective potential property.

背景

本研究检测了矢车菊素对大鼠酒精性肝损伤的影响。

方法

将24只Wistar大鼠以相同方式分为四组：正常对照组（正常组）、乙醇对照组（EtOH组）、矢车菊素低剂量组（10 mg/kg）和矢车菊素高剂量组（20 mg/kg）。对试验组大鼠口服矢车菊素-10和矢车菊素-20，持续4周，而对对照组大鼠给予5 mL/kg蒸馏水。此外，在上述口服治疗的最后一剂后1小时，腹腔注射5 mL/kg乙醇以诱导肝损伤。乙醇处理5小时后，采集血样进行生化检测。

结果

两种剂量的矢车菊素给药均恢复了所有检测的血清指标，即肝功能试验（ALT、AST、ALP）、生化试验（肌酐、白蛋白、BUN、直接胆红素和LDH）、脂质评估（TC和TG）、内源性抗氧化剂（GSH-Px、SOD和CAT）、丙二醛（MDA）、一氧化氮（NO）、细胞因子（TGF-β、TNF-α、IL-1β、IL-6、IFN-γ和IL-12）、半胱天冬酶-3和与乙醇组相关的核因子κB（NF-κB）。