Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China.
Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.
PeerJ. 2023 Jun 27;11:e15641. doi: 10.7717/peerj.15641. eCollection 2023.
MicroRNA-154-5p (miR-154-5p) plays a role in tumorigenesis in diverse human malignancies. Nevertheless, little is known about the mechanism by which miR-154-5p alters the growth and metastasis of cervical cancer. This research aimed to analyze the role of miR-154-5p in the pathology of cervical cancer and .
The level of miR-154-5p in human papillomavirus 16 positive cervical cancer cells was examined by real-time quantitative polymerase chain reaction. Bioinformatics predicted the downstream targets and potential functions of miR-154-5p. Furthermore, lentiviral technology was used to construct SiHa cell lines with stable up- and down-expression levels of miR-154-5p. Its differential expression effects on the progress and metastasis of cervical cancer were analyzed using cell culture and animal models.
MiR-154-5p showed low expression in cervical cancer cells. Overexpression of miR-154-5p could markedly inhibit the proliferation, migration, and colony formation ability of SiHa cells, concomitantly leading to G1 arrest of the cell cycle, while silencing miR-154-5p triggered the opposite results. Meanwhile, overexpression of miR-154-5p restrained the growth and metastasis of cervical cancer by silencing CUL2 . Additionally, miR-154-5p reduced CUL2 level, and overexpression of CUL2 influenced the effect of miR-154-5p in cervical cancer. In conclusion, miR-154-5p restrained the growth and metastasis of cervical cancer by directly silencing CUL2.
MicroRNA-154-5p（miR-154-5p）在多种人类恶性肿瘤的发生中起作用。然而，miR-154-5p 如何改变宫颈癌的生长和转移机制知之甚少。本研究旨在分析 miR-154-5p 在宫颈癌中的作用。
采用实时定量聚合酶链反应检测人乳头瘤病毒 16 阳性宫颈癌细胞中 miR-154-5p 的水平。生物信息学预测了 miR-154-5p 的下游靶标和潜在功能。此外，采用慢病毒技术构建 miR-154-5p 稳定过表达和低表达的 SiHa 细胞系。利用细胞培养和动物模型分析其对宫颈癌进展和转移的差异表达效应。
miR-154-5p 在宫颈癌细胞中表达较低。过表达 miR-154-5p 可显著抑制 SiHa 细胞的增殖、迁移和集落形成能力，同时导致细胞周期 G1 期阻滞，而沉默 miR-154-5p 则引发相反的结果。同时，过表达 miR-154-5p 通过沉默 CUL2 抑制宫颈癌的生长和转移。此外，miR-154-5p 降低了 CUL2 的水平，而过表达 CUL2 影响 miR-154-5p 在宫颈癌中的作用。综上所述，miR-154-5p 通过直接沉默 CUL2 抑制宫颈癌的生长和转移。
