Horisawa Kenichi, Suzuki Atsushi
Division of Organogenesis and Regeneration, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
Regen Ther. 2023 Jun 19;24:112-116. doi: 10.1016/j.reth.2023.06.002. eCollection 2023 Dec.
Regenerative medicine is a highly advanced medical field that aims to restore tissues and organs lost due to diseases and injury using a person's own cells or those of others. Direct cellular reprogramming is a promising technology that can directly induce cell-fate conversion from terminally differentiated cells to other cell types and is expected to play a pivotal role in applications in regenerative medicine. The induction of direct cellular reprogramming requires one or more master transcription factors with the potential to reconstitute cell type-specific transcription factor networks. The set of master transcription factors may contain unique transcription factors called pioneer factors that can open compacted chromatin structures and drive the transcriptional activation of target genes. Therefore, pioneer factors may play a central role in direct cellular reprogramming. However, our understanding of the molecular mechanisms by which pioneer factors induce cell-fate conversion is still limited. This review briefly summarizes the outcomes of recent findings and discusses future perspectives, focusing on the role of pioneer factors in direct cellular reprogramming.
再生医学是一个高度先进的医学领域，旨在利用一个人自身的细胞或他人的细胞来恢复因疾病和损伤而丧失的组织和器官。直接细胞重编程是一项很有前景的技术，它可以直接诱导终末分化细胞向其他细胞类型的细胞命运转变，并有望在再生医学应用中发挥关键作用。直接细胞重编程的诱导需要一种或多种具有重构细胞类型特异性转录因子网络潜力的主转录因子。主转录因子组可能包含称为先驱因子的独特转录因子，这些先驱因子可以打开紧密的染色质结构并驱动靶基因的转录激活。因此，先驱因子可能在直接细胞重编程中发挥核心作用。然而，我们对先驱因子诱导细胞命运转变的分子机制的理解仍然有限。本综述简要总结了近期研究结果，并讨论了未来展望，重点关注先驱因子在直接细胞重编程中的作用。
