Zhang Yu-Wen, Shi Yu-Cen, Zhang Shi-Bao
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China.
Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, Yunnan, China.
Plant Divers. 2022 Oct 27;45(3):326-336. doi: 10.1016/j.pld.2022.10.004. eCollection 2023 May.
is an important medicinal and nutraceutical herb. Although the ingredients of have been identified as polysaccharides, alkaloids, amino acids, flavonoids and bibenzyls, our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited. Here, we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of . A total of 1005 metabolites and 31,745 genes were detected in the stems of . The majority of these metabolites and genes were involved in the metabolism of carbohydrates (fructose, mannose, glucose, xylulose and starch), while some were involved in the metabolism of secondary metabolites (alkaloids, β-tyrosine, ferulic acid, 4-hydroxybenzoate and chrysin). Our predicted regulatory network indicated that five genes (, , , and ) might play vital roles in the transition from carbohydrate to alkaloid synthesis. Correlation analysis identified that six genes (, , , , and ) were involved in carbohydrate metabolism, and two genes ( and ) were involved in secondary metabolite biosynthesis. Our analyses also indicated that phosphoenol-pyruvate (PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis. The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in species.
是一种重要的药用和营养草本植物。尽管已确定其成分包括多糖、生物碱、氨基酸、黄酮类化合物和联苄，但我们对调节这些化合物合成的代谢途径的了解有限。在此，我们利用转录组学和代谢分析来阐明参与其茎中碳水化合物和几种次生代谢物生物合成的基因和代谢物。在其茎中总共检测到1005种代谢物和31745个基因。这些代谢物和基因中的大多数参与碳水化合物（果糖、甘露糖、葡萄糖、木酮糖和淀粉）的代谢，而一些则参与次生代谢物（生物碱、β - 酪氨酸、阿魏酸、4 - 羟基苯甲酸和白杨素）的代谢。我们预测的调控网络表明，五个基因（，，，和）可能在从碳水化合物合成向生物碱合成的转变中起关键作用。相关性分析确定六个基因（，，，，和）参与碳水化合物代谢，两个基因（和）参与次生代谢物生物合成。我们的分析还表明，磷酸烯醇丙酮酸（PEP）是连接碳水化合物和生物碱生物合成的关键桥梁。所建立的碳水化合物和次生代谢物生物合成之间的调控网络将为该物种中代谢物和生物系统的调控提供重要见解。
