Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, People's Republic of China.
Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.
Mol Biol Rep. 2019 Oct;46(5):5175-5184. doi: 10.1007/s11033-019-04975-5. Epub 2019 Jul 16.
Isoprenoids, the largest and most diverse class of secondary metabolites in plants, play an important role in plant growth and development. Isoprenoids can be synthesized by two distinct pathways: the methylerythritol-4-phosphate (MEP) pathway and the mevalonate (MVA) pathway. 1-Deoxy-D-xylulose-5-phosphate synthase (DXS) is the first step and a key regulatory enzyme of the MEP pathway in plants. The DXS gene has been reported to play a key role in seedling development, flowering, and fruit quality in plants of the Solanaceae, such as tomato, potato and tobacco. However, to improve our understanding and utilization of DXS genes, a thorough bioinformatics study is needed. In this study, 48 DXS genes were aligned and analyzed by computational tools to predict their protein properties, including molecular mass, theoretical isoelectric point (pI), signal peptides, transmembrane and conserved domains, and expression patterns. Sequence comparison analysis revealed strong conservation among the 48 DXS genes. Phylogenetic analysis indicated that all DXS genes were derived from one ancestor and could be classified into three groups with different expression patterns. Moreover, the functional divergence of DXS was restricted after gene duplication. The results suggested that the function and evolution of the DXS gene family were highly conserved and that the DXS genes of Group I may play a more important role than those of other groups.
类异戊二烯是植物中最大和最多样化的次生代谢物类别，在植物生长和发育中起着重要作用。类异戊二烯可以通过两条不同的途径合成：1-脱氧-D-木酮糖-5-磷酸合酶（DXS）是植物 MEP 途径的第一步和关键调节酶。已报道 DXS 基因在茄科植物（如番茄、土豆和烟草）的幼苗发育、开花和果实品质中发挥关键作用。然而，为了提高我们对 DXS 基因的理解和利用，需要进行全面的生物信息学研究。在这项研究中，通过计算工具对 48 个 DXS 基因进行了比对和分析，以预测其蛋白质特性，包括分子量、理论等电点（pI）、信号肽、跨膜和保守结构域以及表达模式。序列比较分析表明，48 个 DXS 基因之间存在很强的保守性。系统发育分析表明，所有 DXS 基因都源自一个共同的祖先，并且可以根据不同的表达模式分为三组。此外，DXS 的功能分化在基因复制后受到限制。结果表明，DXS 基因家族的功能和进化高度保守，并且第一组的 DXS 基因可能比其他组的基因发挥更重要的作用。
