Qiu Qin, Liu Xiaofang, Huang Chunying, Guo Yanling, Zhen Dandan, Shi Junhao, Gu Baojun, Zhen Hanshen, Zhang Miao
Guangxi University of Chinese Medical, Nanning 530200, China.
Traditional Chinese Medicine Hospital of YuLin, Yulin 537099, China.
J Anal Methods Chem. 2023 Jun 23;2023:1242756. doi: 10.1155/2023/1242756. eCollection 2023.
Benth. () is one of the most frequently utilized traditional Chinese medicine in Zhuang medicine. Simultaneously, it has been included in the "Quality Standard of Zhuang medicine in Guangxi Zhuang Autonomous Region (Vol. 2)" and possessed outstanding pharmacological effects. However, the pharmacodynamic material basis of still remains unclear. In this study, the high-performance liquid chromatography (HPLC) method had been employed to establish the fingerprint of 10 batches of aqueous extract of originated from different parts of China. At the same time, similarity evaluation, cluster analysis, and principal component analysis (PCA) had also been conducted to evaluate the common peaks. The acetic acid-induced writhing in mice had been employed as an analgesic model, and the carrageenan-induced toe swelling in mice was utilized as an anti-inflammatory model for pharmacodynamic experiments. The gray relational analysis (GRA) and partial least squares regression (PLSR) were applied to correlate the fingerprint and pharmacodynamic data to thoroughly examine its spectrum-effect relationship, whereby its analgesic and anti-inflammatory material basis had been comprehensively explored. The results revealed that the HPLC fingerprint of the aqueous extract of had successfully identified 12 common peaks whereby two of which were further identified as protocatechuic acid and vitexin. Subsequently, through the analysis of GRA and PLSR, the chromatographic peaks that possess a critical correlation degree with the analgesic and anti-inflammatory effects of had also been successfully discovered. Ultimately, the analgesic and anti-inflammatory effects of the 10 batches of aqueous extract had been conclusively proved, and it was evidently indicated that these effects were attributable to the synergistic interactions between various components. Therefore, this study aims to serve as an effective analytical method for screening and predicting the effective substances of traditional Chinese medicine on the basis of the spectrum-effect relationship.
（文中未提及具体植物名称，暂用“某植物”指代）是壮药中最常用的传统中药之一。同时，它已被列入《广西壮族自治区壮药质量标准（第二卷）》，并具有显著的药理作用。然而，某植物的药效物质基础仍不清楚。在本研究中，采用高效液相色谱（HPLC）法建立了来自中国不同地区的10批次某植物水提取物的指纹图谱。同时，还进行了相似度评价、聚类分析和主成分分析（PCA）以评估共有峰。采用醋酸诱导的小鼠扭体反应作为镇痛模型，角叉菜胶诱导的小鼠足趾肿胀作为抗炎模型进行药效学实验。应用灰色关联分析（GRA）和偏最小二乘回归（PLSR）将指纹图谱与药效学数据进行关联，全面考察其谱效关系，从而全面探索其镇痛和抗炎物质基础。结果表明，某植物水提取物的HPLC指纹图谱成功鉴定出12个共有峰，其中两个进一步鉴定为原儿茶酸和牡荆素。随后，通过GRA和PLSR分析，也成功发现了与某植物镇痛和抗炎作用具有关键关联度的色谱峰。最终，确凿地证明了10批次某植物水提取物的镇痛和抗炎作用，并且明显表明这些作用归因于各种成分之间的协同相互作用。因此，本研究旨在为基于谱效关系筛选和预测中药有效物质提供一种有效的分析方法。
