El-Diasty Mohamed, Wareth Gamal, Melzer Falk, Mustafa Shawky, Sprague Lisa D, Neubauer Heinrich
Animal Health Research Institute-Mansoura Provincial Laboratory, 35516 Mansoura, Egypt.
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, 07743 Jena, Germany.
Vet Sci. 2018 Mar 9;5(1):28. doi: 10.3390/vetsci5010028.
Brucellosis is a zoonosis occurring worldwide, with economic and public health impacts. Its diagnosis remains a challenge in endemic countries and basically relies on serology. The present study was carried out on two dairy cattle farms allegedly free from brucellosis, but with sporadic cases of abortion. The aim of this study was to investigate the presence of (B.) spp. in uterine discharge of seronegative cows after abortion. In farm I, biovar (bv) 3 was cultured from two of five cows after abortion, while in farm II, bv 1 was cultured from three of eleven cows after abortion. These cows had been intrauterinely infected but remained seronegative until abortion and seroconverted only thereafter. Shedding of brucellae in uterine discharge of culture positive/seronegative aborting cows is a serious problem resulting in maintenance and further spread of infection. Thus, serosurveys in endemic countries have to be accompanied by molecular detection and/or culture of aborted material to close the diagnostic window and to hinder uncontrolled spread.
布鲁氏菌病是一种在全球范围内发生的人畜共患病，对经济和公共卫生有影响。在流行国家，其诊断仍然是一项挑战，基本上依赖血清学检测。本研究在两个据称无布鲁氏菌病但有散发性流产病例的奶牛场进行。本研究的目的是调查流产后血清学阴性奶牛子宫分泌物中布鲁氏菌属（B.）的存在情况。在农场I，从五头流产奶牛中的两头培养出生物变种（bv）3，而在农场II，从十一头流产奶牛中的三头培养出bv 1。这些奶牛曾经子宫内感染，但在流产前一直保持血清学阴性，仅在流产后才发生血清转化。培养阳性/血清学阴性的流产奶牛子宫分泌物中布鲁氏菌的排出是一个严重问题，会导致感染的持续存在和进一步传播。因此，在流行国家进行血清学调查时，必须同时进行分子检测和/或对流产材料进行培养，以缩小诊断窗口期并防止感染的无控制传播。
