China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University, Beijing 100048, PR China.
Diet Genomics and Immunology Laboratory, BHNRC, ARS, USDA, Beltsville, MD 20705, USA.
Food Funct. 2023 Jul 17;14(14):6654-6664. doi: 10.1039/d3fo01249b.
Cruciferous vegetable microgreens, such as red cabbage microgreens (RCMG), are of special interest due to their well-documented health-promoting effects compared to their mature counterparts. However, little is known of the biological effects of microgreens. The present study used a rodent diet-induced obesity model to investigate the effect of consuming RCMG on the gut microbiota. We found that the consumption of RCMG exerted profound impacts on the microbial composition in mice. Specifically, the species diversity of mice on both low fat (LF) and high fat (HF) diets was significantly increased by the consumption of RCMG. In comparison with the LF control group, the intake of RCMG increased the gut Firmicutes/Bacteroidetes (F/B) ratio. Furthermore, an unidentified species of the Clostridiales order, increased by RCMG, was found to be negatively correlated with the hepatic cholesterol ester level in mice ( = -0.43, < 0.05). In addition, RCMG significantly inhibited HF diet-induced elevation of the genus , of which the abundance was positively correlated with the body weight gain ( = 0.52, < 0.01) and fecal bile acid in mice ( = 0.59, < 0.01). Overall, our results demonstrated that the consumption of RCMG in the diet can alter the gut microbiota, and attenuation of HF diet-induced body weight gain and altered cholesterol metabolism may be mediated through regulation of the gut microbiota.
十字花科蔬菜芽苗菜，如红甘蓝芽苗菜（RCMG），由于其与成熟蔬菜相比具有良好的促进健康的作用而备受关注。然而，对于芽苗菜的生物学效应却知之甚少。本研究采用了一种啮齿动物饮食诱导肥胖模型来研究食用 RCMG 对肠道微生物群的影响。我们发现，食用 RCMG 对小鼠肠道微生物组成产生了深远的影响。具体来说，RCMG 的摄入显著增加了低脂（LF）和高脂（HF）饮食小鼠的物种多样性。与 LF 对照组相比，RCMG 的摄入增加了肠道厚壁菌门/拟杆菌门（F/B）的比例。此外，研究发现，RCMG 增加的梭状芽胞杆菌目未鉴定物种与小鼠肝脏胆固醇酯水平呈负相关（ = -0.43，<0.05）。此外，RCMG 显著抑制了 HF 饮食诱导的属的增加，其丰度与小鼠体重增加呈正相关（ = 0.52，<0.01）和粪便胆汁酸呈正相关（ = 0.59，<0.01）。总的来说，我们的结果表明，饮食中 RCMG 的摄入可以改变肠道微生物群，而 HF 饮食诱导的体重增加和胆固醇代谢的改变可能是通过调节肠道微生物群来介导的。
