利用新型多酶等温快速检测法快速检测结核分枝杆菌中的氟喹诺酮耐药性。
Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay.
机构信息
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Department of Laboratory Medicine, Ningbo First Hospital, Ningbo Hospital of Ningbo University, Ningbo, 315010, China.
出版信息
J Antibiot (Tokyo). 2023 Oct;76(10):598-602. doi: 10.1038/s41429-023-00639-6. Epub 2023 Jul 4.
Simple, rapid, and accurate detection of Fluoroquinolone (FQ) resistance is essential for early initiation of appropriate anti-tuberculosis treatment regimen among rifampicin-resistant tuberculosis (RR-TB). In this study, we developed a new assay, which combines multienzyme isothermal rapid amplification and a lateral flow strip (MIRA-LF), to identify the mutations on codons 90 and 94 of gyrA for detecting levofloxacin (LFX) resistance. Compared to conventional phenotypic drug susceptibility testing, the new assay detected fluoroquinolone resistance with a sensitivity, specificity, and accuracy of 92.4%, 98.5%, and 96.5%, respectively. Thus, these characteristics of the newly developed MIRA-LF assay make it particularly useful and accurate for detecting FQ resistance in Mycobacterium tuberculosis in resource-limited condition.
快速、准确地检测氟喹诺酮（FQ）耐药性对于在利福平耐药结核病（RR-TB）中尽早开始适当的抗结核治疗方案至关重要。在这项研究中，我们开发了一种新的检测方法，即多酶等温快速扩增和侧向流条（MIRA-LF）结合，以确定 gyrA 密码子 90 和 94 上的突变，用于检测左氧氟沙星（LFX）耐药性。与传统的表型药敏试验相比，新的检测方法检测氟喹诺酮耐药性的敏感性、特异性和准确性分别为 92.4%、98.5%和 96.5%。因此，新开发的 MIRA-LF 检测方法的这些特点使其在资源有限的情况下特别有用且准确地检测结核分枝杆菌中的 FQ 耐药性。
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