Fan Lijiao, Wei Dongshan, Yu Xingwang, Yu Fengqiang, Wang Jiameng, Sun Guirong, Zhang Li, Zhang Guosheng, Yang Haifeng
College of Forestry, Inner Mongolia Agricultural University, Hohhot 010018, China.
Development Center of Forestry and Grassland, Ordos 017000, China.
Breed Sci. 2023 Apr;73(2):180-192. doi: 10.1270/jsbbs.22079. Epub 2023 Apr 13.
To identify the function of the gene and its response to salt and drought stress, the gene was transformed into by the Agrobacterium-mediated leaf disc method, and the phenotypic, physiological changes and related genes expression of transgenic lines were analyzed. The results showed that the number and length of roots of transgenic lines increased significantly. The leaves of transgenic lines curled inward. Under salt and simulated drought stress, the transgenic lines showed improved tolerance to salt and drought. The activities of SOD, POD, CAT and proline content in the transgenic lines were significantly increased, and the reduction rates of total chlorophyll and MDA content were significantly decreased, which indicated that the transgenic lines showed strong physiological responses under stress. Meanwhile, the gene expression of , , and were significantly upregulated, and the gene expression of was significantly downregulated, which preliminarily verified the stress regulation mechanism that might activate. The above results showed that the gene could promote root development, make leaf morphology curl, and enhance tolerance to stress.
为了鉴定该基因的功能及其对盐和干旱胁迫的响应，通过农杆菌介导的叶盘法将该基因转化到[具体受体]中，并分析了转基因株系的表型、生理变化及相关基因表达。结果表明，转基因株系的根数量和长度显著增加。转基因株系的叶片向内卷曲。在盐和模拟干旱胁迫下，转基因株系对盐和干旱的耐受性增强。转基因株系中SOD、POD、CAT活性和脯氨酸含量显著增加，总叶绿素和MDA含量的降低率显著降低，这表明转基因株系在胁迫下表现出强烈的生理响应。同时，[相关基因名称1]、[相关基因名称2]、[相关基因名称3]和[相关基因名称4]的基因表达显著上调，而[相关基因名称5]的基因表达显著下调，这初步验证了[该基因名称]可能激活的胁迫调控机制。上述结果表明，[该基因名称]基因可促进根系发育，使叶片形态卷曲，并增强[受体名称]对胁迫的耐受性。
