Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000, Versailles, France
Development. 2020 Mar 18;147(6):dev183277. doi: 10.1242/dev.183277.
How a shape arises from the coordinated behavior of cells is one of the most fascinating questions in developmental biology. In plants, fine spatial and temporal controls of cell proliferation and cell expansion sustain differential growth that defines organ shape and size. At the leaf margin of , interplay between auxin transport and transcription factors named CUP SHAPED COTYLEDON (CUCs), which are involved in the establishment of boundary domain identity, were reported to trigger differential growth, leading to serration. Cellular behaviors behind these differential growths remain scarcely described. Here, we used 3D and time lapse imaging on young leaves at different stages of development to determine the sequence of cellular events resulting in leaf serrations. In addition, we showed that the transcription factor CUC3 is a negative regulator of cell growth and that its expression dynamics in a small number of cells at the leaf margin is tightly associated with the control of differential growth.
细胞的协调行为如何产生形状是发育生物学中最吸引人的问题之一。在植物中，细胞增殖和细胞扩展的精细时空控制维持了决定器官形状和大小的差异生长。在 的叶边缘，生长素运输和转录因子（命名为 CUP SHAPED COTYLEDON，CUCs）之间的相互作用被报道可以触发差异生长，导致锯齿状。这些差异生长背后的细胞行为仍然很少被描述。在这里，我们使用 3D 和延时成像技术在不同发育阶段的幼叶上，以确定导致叶片锯齿状的细胞事件的顺序。此外，我们还表明，转录因子 CUC3 是细胞生长的负调节剂，其在叶边缘少数细胞中的表达动态与差异生长的控制密切相关。
