Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-612, Poland.
Department of Preventive Veterinary and Avian Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-950, Poland.
Poult Sci. 2023 Sep;102(9):102823. doi: 10.1016/j.psj.2023.102823. Epub 2023 May 29.
The aim of the study was to determine the effect of in ovo administration of zinc glycine chelate (Zn-Gly), and a multistrain probiotic on the hatchability and selected parameters of the cellular and humoral immune response of chickens. The study was conducted on 1,400 fertilized eggs from commercial broiler breeders (Ross x Ross 708). Material for the study consisted of peripheral blood and spleens of chicks taken 12 h and 7 d after hatching. The results showed that both combined and single in ovo administration of the multistrain probiotic and zinc glycine chelate significantly reduced hatchability of chicks. The flow cytometry study showed that the highest percentage of CD4 T cells, CD4CD25, and high expression of KUL01 in the serum were obtained in the group supplemented with probiotic and Zn-Gly both 12 h and 7 d after hatching. In birds supplemented with probiotic and zinc chelate, a high percentage of TCRγδ cells was found in serum and spleen 12 h after hatching and in serum after 7 d. The percentage of Bu-1A lymphocytes in serum and spleen 12 h and 7 d after hatching was the highest in the group supplemented with probiotic and Zn-Gly. The highest expression of CD79A was observed in the group supplemented only with zinc chelate. There were no significant differences in the percentage of CD4 cells in the spleens of birds in the groups receiving the multistrain probiotic at 12 h after hatching, and after 7 d, the percentage of CD4 T cells was lower in the experimental groups than in the control group. The percentage of CD8 cells in the serum of birds after hatching was lower in the group supplemented with multistrain probiotic and Zn-Gly than in the control group, but reached the highest value on d 7 after hatching. The obtained results confirm the strong effect of the combined administration of a multistrain probiotic and Zn-Gly chelate on lymphocyte proliferation and stimulation of cellular immune mechanisms in birds.
本研究旨在确定卵内给予甘氨酸锌螯合物（Zn-Gly）和多菌株益生菌对孵化率和鸡细胞和体液免疫应答的选择参数的影响。该研究在来自商业肉鸡品种（Ross x Ross 708）的 1400 枚受精卵上进行。研究材料包括孵化后 12 小时和 7 天的雏鸡外周血和脾脏。结果表明，多菌株益生菌和甘氨酸锌螯合物的联合和单独卵内给药均显著降低了雏鸡的孵化率。流式细胞术研究表明，在补充益生菌和 Zn-Gly 的组中，12 小时和 7 天后孵化时血清中 CD4 T 细胞、CD4CD25 和 KUL01 的高表达率最高。在补充益生菌和锌螯合物的鸟类中，在孵化后 12 小时在血清和脾脏中以及在孵化后 7 天在血清中发现 TCRγδ 细胞的百分比较高。在补充益生菌和 Zn-Gly 的组中，在孵化后 12 小时和 7 天后的血清和脾脏中，Bu-1A 淋巴细胞的百分比最高。仅补充锌螯合物时观察到 CD79A 的表达最高。在孵化后 12 小时，接受多菌株益生菌的各组鸡脾脏中 CD4 细胞的百分比没有差异，而在 7 天后，实验组 CD4 T 细胞的百分比低于对照组。孵化后鸟类血清中 CD8 细胞的百分比在补充多菌株益生菌和 Zn-Gly 的组中低于对照组，但在孵化后第 7 天达到最高值。获得的结果证实了多菌株益生菌和 Zn-Gly 螯合物联合给药对禽类淋巴细胞增殖和刺激细胞免疫机制的强烈影响。
