Jarosz Łukasz, Marek Agnieszka, Grądzki Zbigniew, Kwiecień Małgorzata, Kalinowski Marcin
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland; Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
Res Vet Sci. 2017 Jun;112:59-65. doi: 10.1016/j.rvsc.2016.09.007. Epub 2016 Sep 14.
The ability of poultry to withstand infectious disease caused by bacteria, viruses or protozoa depends upon the integrity of the immune system. Zinc is important for proper functioning of heterophils, mononuclear phagocytes and T lymphocytes. Numerous data indicate that the demand for zinc in poultry is not met in Poland due to its low content in feeds of vegetable origin. The aim of the study was to determine the effect of supplementation of inorganic (ZnSO and ZnSO phytase enzyme), and organic forms of zinc (Zn with glycine and Zn with glycine and phytase enzyme) on selected parameters of the cellular and humoral immune response in broiler chickens by evaluating the percentage of CD3CD4, CD3CD8, CD25, MHC Class II, and BU-1 lymphocytes, the phagocytic activity of monocytes and heterophils, and the concentration of IL-2, IL-10 and TNF-α in the peripheral blood. Flow cytometry was used to determine selected cell-mediated immune response parameters. Phagocytic activity in whole blood was performed using the commercial Phagotest kit (ORPEGEN-Pharma, Immuniq, Poland). The results showed that supplementation with zinc chelates causes activation of the cellular and humoral immune response in poultry, helping to maintain the balance between the Th1 and Th2 response and enhancing resistance to infections. In contrast with chelates, the use of zinc in the form of sulphates has no immunomodulatory effect and may contribute to the development of local inflammatory processes in the digestive tract, increasing susceptibility to infection.
家禽抵御由细菌、病毒或原生动物引起的传染病的能力取决于免疫系统的完整性。锌对于嗜异性粒细胞、单核吞噬细胞和T淋巴细胞的正常功能很重要。大量数据表明，由于波兰植物性饲料中锌含量低，家禽对锌的需求未得到满足。本研究的目的是通过评估CD3CD4、CD3CD8、CD25、MHC II类和BU-1淋巴细胞的百分比、单核细胞和嗜异性粒细胞的吞噬活性以及外周血中IL-2、IL-10和TNF-α的浓度，来确定补充无机锌（ZnSO和ZnSO植酸酶）和有机锌（甘氨酸锌和甘氨酸锌与植酸酶）对肉鸡细胞免疫和体液免疫反应选定参数的影响。使用流式细胞术来确定选定的细胞介导免疫反应参数。使用商业Phagotest试剂盒（ORPEGEN-Pharma，Immuniq，波兰）检测全血中的吞噬活性。结果表明，补充锌螯合物可激活家禽的细胞免疫和体液免疫反应，有助于维持Th1和Th2反应之间的平衡，并增强对感染的抵抗力。与螯合物相比，使用硫酸盐形式的锌没有免疫调节作用，可能会导致消化道局部炎症过程的发展，增加感染易感性。
