ICAR - National Institute of Animal Nutrition and Physiology, Bangalore, India.
J Anim Physiol Anim Nutr (Berl). 2018 Apr;102(2):591-595. doi: 10.1111/jpn.12777. Epub 2017 Oct 8.
An experiment was conducted to determine the effect of in ovo administration of different forms of zinc with respect to hatchability and performance of commercial broiler chicken. In trial 1, the fertile eggs on day 18 were divided into six treatment groups: Group I as control without any supplementation of zinc, group II to IV were supplemented with 0.5 mg zinc per egg as zinc sulphate, zinc methionine or nano zinc, respectively, and Group V with nano zinc at 0.25 mg zinc per egg. Sixth group received 0.5 ml citric acid per egg as sham control. The results of the first trial indicated that in ovo administration of nano zinc at both levels and zinc methionine resulted in complete failure of hatchability. A second trial to validate the result of trial 1 consisted of Group I control (no administration). Group II and Group III were supplemented with zinc sulphate and zinc methionine, respectively, at 0.5 mg zinc per egg. Group IV and Group V were supplemented with nano zinc at 0.04 and 0.08 mg per egg. In the second trial, again there was a similar pattern for zinc sulphate and zinc methionine. Administration of Zn by nano form had around 80% hatchability on fertile eggs in comparison with the unadministered control eggs (92%). There was no difference (p > .05) in body weight gain, feed intake and FCR. No difference (p > .05) was observed between treatments for cell-mediated immune response and humoral immune response. Nano Zn-administered group showed a non-significant downregulation of MUC2 gene. It could be concluded that in ovo administration of higher levels of zinc has to be with caution for the developing embryo of commercial broiler chicken.
一项实验旨在确定在鸡胚中添加不同形式的锌对孵化率和商业肉鸡性能的影响。在试验 1 中，将第 18 天的受精卵分为 6 个处理组：第 1 组为对照组，不添加任何锌；第 2 组至第 4 组分别以硫酸锌、蛋氨酸锌和纳米锌的形式每枚蛋添加 0.5mg 锌，第 5 组以纳米锌的形式每枚蛋添加 0.25mg 锌。第 6 组接受每枚蛋 0.5ml 柠檬酸作为假对照。第 1 次试验的结果表明，在两种水平下给鸡胚注射纳米锌和蛋氨酸锌都会导致孵化率完全失败。第二次试验旨在验证第 1 次试验的结果，由对照组 1（未给药）组成。第 2 组和第 3 组分别以硫酸锌和蛋氨酸锌的形式每枚蛋添加 0.5mg 锌。第 4 组和第 5 组分别以纳米锌的形式每枚蛋添加 0.04 和 0.08mg 锌。第二次试验中，硫酸锌和蛋氨酸锌也出现了类似的模式。与未给药的对照组鸡蛋（92%）相比，纳米形式的锌给药对可孵化的受精卵的孵化率约为 80%。体重增加、饲料摄入和 FCR 没有差异（p>.05）。细胞介导的免疫反应和体液免疫反应的处理之间没有差异（p>.05）。纳米锌给药组的 MUC2 基因表达下调不明显。可以得出结论，在鸡胚中添加高水平的锌必须谨慎，以免对商业肉鸡的胚胎发育造成影响。
