Zhou Qi, Li Yongping, Wang Xiaojing, Yan Chao, Ma Chunmei, Liu Jun, Dong Shoukun
Agricultural College, Northeast Agricultural University, Harbin 150030, China.
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plants (Basel). 2022 Aug 31;11(17):2282. doi: 10.3390/plants11172282.
Drought affects crop developmentnand growth. To explore the physiological effects of drought stress on soybean, HeiNong44 (HN44) and HeiNong65 (HN65) varieties were used as experimental materials and PEG-6000 was used as the osmotic medium. The antioxidant enzyme activity, osmotic adjustment substance content, antioxidant capacity, and endogenous hormone content of the two soybean varieties were studied under different drought degrees and different treatment durations. Drought stress caused significant physiological changes in soybean. The antioxidant enzyme activities, osmoregulation substance content, and total antioxidant capacity (T-AOC) of HN65 and HN44 showed an increasing trend under mild and moderate drought, however, they first increased and then decreased under severe drought conditions. Following the extension of treatment time, malondialdehyde (MDA) showed an increasing trend. As drought increased, gibberellin (GA) content showed a decreasing trend, while abscisic acid (ABA), salicylic acid (SA), and zeatin nucleoside (ZA) content showed an increasing trend. The auxin (IAA) content of the two varieties showed opposite change trends. In short, drought had a significant impact on the physiology of these two soybean varieties; however, overall, the drought resistance of HN65 was lower than that of HN44. This study provides a research theoretical basis for addressing the drought resistance mechanism and the breeding of drought resistant soybean varieties.
干旱影响作物发育和生长。为探究干旱胁迫对大豆的生理影响，以黑农44（HN44）和黑农65（HN65）品种为试验材料，以聚乙二醇-6000（PEG-6000）作为渗透介质。研究了两个大豆品种在不同干旱程度和不同处理时长下的抗氧化酶活性、渗透调节物质含量、抗氧化能力及内源激素含量。干旱胁迫导致大豆发生显著的生理变化。在轻度和中度干旱条件下，HN65和HN44的抗氧化酶活性、渗透调节物质含量及总抗氧化能力（T-AOC）呈上升趋势，然而在重度干旱条件下则先升高后降低。随着处理时间的延长，丙二醛（MDA）呈上升趋势。随着干旱加剧，赤霉素（GA）含量呈下降趋势，而脱落酸（ABA）、水杨酸（SA）和玉米素核苷（ZA）含量呈上升趋势。两个品种的生长素（IAA）含量呈现相反的变化趋势。总之，干旱对这两个大豆品种的生理有显著影响；然而总体而言，HN65的抗旱性低于HN44。本研究为解析大豆抗旱机制及培育抗旱大豆品种提供了研究理论依据。
