基于体外酶活性的 UHPLC-Q-Orbitrap-HRMS 快速鉴定雪莲颗粒中的化学成分。
Rapid identification of chemical components in Xuelian granule by UHPLC-Q-orbitrap-HRMS based on enzyme activity in vitro.
机构信息
The State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
出版信息
BMC Complement Med Ther. 2023 Jul 5;23(1):222. doi: 10.1186/s12906-023-04025-5.
BACKGROUND
Xuelian granule (XL), a traditional Chinese medicine (TCM) formula, has been used for the treatment of diabetic nephropathy for a long time as a hospital preparation. Because the active ingredients in the XL that can help to treat diabetic nephropathy are still unclear, which limits the interpretation for its pharmacological mechanism, further development and subsequent study on the material basis of its efficacy.
METHODS
In this study, a screening method based on inhibition activity against aldose reductase (AR) was employed for activity-directed chemical analysis of XL using ultra-high performance liquid chromatography combined with quadrupole-orbitrap high resolution mass spectrometry (UHPLC-Q-orbitrap-HRMS) technique.
RESULTS
A total of 178 compounds, including 46 terpenes, 47 organic acids, 25 flavonoids, 29 phenylethanoid glycosides, and 31 other types, were tentatively identified from XL which might responsible for its AR inhibition activity.
CONCLUSION
This is the first study for a systematic, rapid, and accurate qualitative analysis of XL. This research provides a scientific and experimental basis for further researches on pharmacodynamics material basis and quality control of XL.
背景
雪莲颗粒（XL）是一种中药方剂，长期以来一直作为医院制剂用于治疗糖尿病肾病。由于能够治疗糖尿病肾病的 XL 的活性成分仍不清楚，这限制了对其药理机制、进一步开发和随后对其功效物质基础的研究的解释。
方法
本研究采用基于对醛糖还原酶（AR）抑制活性的筛选方法，利用超高效液相色谱-四级杆轨道阱高分辨质谱联用（UHPLC-Q-Orbitrap-HRMS）技术对 XL 进行活性导向化学分析。
结果
从 XL 中初步鉴定出 178 种化合物，包括 46 种萜类、47 种有机酸、25 种黄酮类、29 种苯乙醇苷类和 31 种其他类型，这些化合物可能与其 AR 抑制活性有关。
结论
这是首次对 XL 进行系统、快速、准确的定性分析。本研究为进一步研究 XL 的药效物质基础和质量控制提供了科学实验依据。
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