Maloney Brigid, Ramos Eric Angel, Bennice Chelsea O, Young Frank, Magnasco Marcelo O
Laboratory of Integrative Neuroscience, The Rockefeller University, New York, NY, United States.
Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL, United States.
Front Physiol. 2023 Jun 20;14:1162807. doi: 10.3389/fphys.2023.1162807. eCollection 2023.
The distribution of octopuses within the species complex remains inadequately understood. Species determination can be complex and involves characterizing a specimen's physical features and comparing its genetic makeup to other populations. In this study, we present the first genetic confirmation of (Leite and Haimovici, 2008) inhabiting the coastal waters of the Florida Keys, United States. We employed visual observations to identify species-specific body patterns of three wild-caught octopuses and used genome assembly to confirm their species. All three specimens exhibited a red/white reticulated pattern on their ventral arm surface. Two specimens displayed body pattern components of deimatic display (white eye encircled by a light ring, with darkening around the eye). All visual observations were consistent with distinguishing features of . We then compared mitochondrial subunits COI, COIII, and 16S in these specimens across all available annotated octopod sequences, including (Hotaling et al., 2021) as a control outgroup taxon. For species exhibiting intraspecific genomic variation, we included multiple sequences from geographically distinct populations. Laboratory specimens consistently clustered into a single taxonomic node with . These findings confirm presence in South Florida and suggest a more extensive northern distribution than previously assumed. Whole genome Illumina sequencing of multiple specimens enabled taxonomic identification with well-established DNA barcodes while also generating the first full assembly of . Furthermore, constructing and comparing phylogenetic trees for multiple conserved genes is essential for confirming the presence and delineation of cryptic species in the Caribbean.
在这个物种复合体中，章鱼的分布情况仍未得到充分了解。物种鉴定可能很复杂，涉及描述标本的物理特征，并将其基因组成与其他种群进行比较。在本研究中，我们首次通过基因确认了（莱特和海莫维奇，2008年）生活在美国佛罗里达群岛沿海水域。我们通过视觉观察来识别三只野生捕获章鱼的物种特异性身体图案，并使用基因组组装来确认它们的物种。所有三个标本在其腹侧腕表面都呈现出红/白网状图案。两个标本展示了恐吓展示的身体图案组成部分（白色眼睛被一个亮环包围，眼睛周围颜色变深）。所有视觉观察结果都与该物种的鉴别特征一致。然后，我们将这些标本中的线粒体亚基COI、COIII和16S与所有可用的已注释章鱼序列进行比较，包括将（霍塔林等人，2021年）作为对照外群分类单元。对于表现出种内基因组变异的物种，我们纳入了来自地理上不同种群的多个序列。实验室标本始终与该物种聚集在一个单一的分类节点中。这些发现证实了该物种在南佛罗里达的存在，并表明其分布范围比之前假设的更向北广泛。对多个标本进行全基因组Illumina测序，不仅能够通过成熟的DNA条形码进行分类鉴定，还能首次完成该物种的全基因组组装。此外，构建和比较多个保守基因的系统发育树对于确认加勒比地区隐存物种的存在和界定至关重要。
