Teng Yun, Li Zhige, Liu Junsong, Teng Lesheng, Li Hongdong
State Key Lab of Superhard Materials, College of Physics, Jilin University, Changchun, 130012, People's Republic of China.
School of Life Sciences, Jilin University, Changchun, 130012, People's Republic of China.
Med Oncol. 2023 Jul 6;40(8):227. doi: 10.1007/s12032-023-02096-5.
Blue light (BL) irradiation has been a potentially efficient treatment for many kinds of tumors. In this study, a BL irradiation (centered at 453 nm in wavelength) was proposed to treat the common human liver cancer cell lines of SMMC-7721 and HepG2, examined by means of flow cytometry, western blot, fluorescence microscope assay. In comparison to control groups, the apoptosis and proliferation inhibition of both BL-treated cells are expressively enhanced by mitochondrial apoptosis. The mechanism of apoptosis is related to the more production of reactive oxygen species (ROS) induced by BL and the corresponding changes in the expression of apoptosis-related Bcl-2, Bax and Bad proteins. In addition, the migration rate of the cancer cells could be reduced after BL irradiation. These results demonstrate that introducing BL irradiation is helpful to establish an effective and low toxicity strategy for the clinical treatment of liver tumors.
蓝光（BL）照射已成为多种肿瘤潜在的有效治疗方法。在本研究中，提出了一种蓝光照射（波长以453nm为中心）来治疗人常见肝癌细胞系SMMC - 7721和HepG2，并通过流式细胞术、蛋白质免疫印迹法、荧光显微镜检测进行研究。与对照组相比，经蓝光处理的细胞的凋亡和增殖抑制通过线粒体凋亡显著增强。凋亡机制与蓝光诱导的活性氧（ROS）生成增加以及凋亡相关的Bcl - 2、Bax和Bad蛋白表达的相应变化有关。此外，蓝光照射后癌细胞的迁移率可降低。这些结果表明，引入蓝光照射有助于建立一种有效且低毒的肝肿瘤临床治疗策略。
