Klindworth Daryl L, Salsman Evan, Underdahl Jesse, Elias Elias M, Green Andrew J, Dykes Linda, Faris Justin D, Xu Steven S
USDA-ARS, Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Centre, 1616 Albrecht Blvd, Fargo, ND, 58102-2765, USA.
Department of Plant Sciences, North Dakota State University, Dep. 7670, P.O. Box 6050, Fargo, ND, 58108, USA.
Theor Appl Genet. 2023 Jul 6;136(7):168. doi: 10.1007/s00122-023-04409-8.
Yield and quality tests of wheat lines derived from RWG35 show they carry little, or no linkage drag and are the preferred source of Sr47 for stem rust resistance. Three durum wheat (Triticum turgidum L. subsp. durum) lines, RWG35, RWG36, and RWG37 carrying slightly different Aegilops speltoides introgressions, but each carrying the Sr47 stem rust resistance gene, were backcrossed to three durum and three hard red spring (HRS) wheat (Triticum aestivum L.) cultivars to produce 18 backcross populations. Each population was backcrossed to the recurrent parent six times and prepared for yield trials to test for linkage drag. Lines carrying the introgression (S-lines) were compared to euploid sibling lines (W-lines) and their parent. Yield trials were conducted from 2018 to 2021 at three locations. Three agronomic and several quality traits were studied. In durum, lines derived from RWG35 had little or no linkage drag. Lines derived from RWG36 and RWG37 still retained linkage drag, most notably involving yield and thousand kernel weight, but also test weight, falling number, kernel hardness index, semolina extract, semolina protein content, semolina brightness, and peak height. In HRS wheat, the results were more complex, though the general result of RWG35 lines having little or no linkage drag and RWG36 and RWG37 lines retaining linkage drag still applied. But there was heterogeneity in the Glenn35S lines, and Linkert lines had problems combining with the Ae. speltoides introgressions. We concluded that introgressions derived from RWG35 either had eliminated linkage drag or any negative effects were minor in nature. We recommend that breeders who wish to incorporate Sr47 into their cultivars should work exclusively with germplasm derived from RWG35.
源自RWG35的小麦品系的产量和品质测试表明，它们携带很少或没有连锁累赘，是抗秆锈病Sr47的首选来源。三个硬粒小麦（Triticum turgidum L. subsp. durum）品系RWG35、RWG36和RWG37携带略有不同的节节麦渗入片段，但每个品系都携带Sr47秆锈病抗性基因，它们与三个硬粒小麦和三个硬红春小麦（Triticum aestivum L.）品种回交，产生了18个回交群体。每个群体与轮回亲本回交六次，并准备进行产量试验以测试连锁累赘。携带渗入片段的品系（S系）与整倍体同胞品系（W系）及其亲本进行比较。2018年至2021年在三个地点进行了产量试验。研究了三个农艺性状和几个品质性状。在硬粒小麦中，源自RWG35的品系几乎没有或没有连锁累赘。源自RWG36和RWG37的品系仍然存在连锁累赘，最明显的是涉及产量和千粒重，还有容重、降落数值、籽粒硬度指数、粗粉提取率、粗粉蛋白质含量、粗粉亮度和峰值高度。在硬红春小麦中，结果更为复杂，尽管RWG35品系几乎没有或没有连锁累赘以及RWG36和RWG37品系存在连锁累赘这一总体结果仍然适用。但是Glenn35S品系存在异质性，Linkert品系与节节麦渗入片段的结合存在问题。我们得出结论，源自RWG35的渗入片段要么消除了连锁累赘，要么任何负面影响在本质上都是轻微的。我们建议希望将Sr47纳入其品种的育种者应专门使用源自RWG35的种质。
