CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia.
Agriculture Victoria Research, Department of Jobs, Precincts and Regions, Agribio, 5 Ring Rd, Bundoora, VIC, 3083, Australia.
Theor Appl Genet. 2022 May;135(5):1541-1550. doi: 10.1007/s00122-022-04052-9. Epub 2022 Feb 23.
Adult plant stem rust resistance locus, QSrGH.cs-2AL, was identified in durum wheat Glossy Huguenot and mendelised as Sr63. Markers closely linked with Sr63 were developed. An F population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F for molecular mapping of adult plant stem rust resistance. Maturity differences among F lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F population was developed through single seed descent method. F and F RILs were tested along with the parents at different locations. The F individual plants and both parents were genotyped using the 90 K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele-specific PCR (KASP) assays and were validated on a set of durum cultivars.
成株期抗秆锈病基因 QSrGH.cs-2AL 被鉴定为硬粒小麦 Glossy Huguenot 的一个基因，并被命名为 Sr63。开发了与 Sr63 紧密连锁的标记。先前澳大利亚的研究中使用 Glossy Huguenot（GH）/Bansi 杂交的 F 群体被推进到 F 世代，用于成株期抗秆锈病的分子作图。F 系之间的成熟度差异使秆锈病反应的评估变得复杂。GH 与感秆锈病的 F 重组自交系（RIL）GHB14（M14）杂交，两者成熟度相似，并通过单粒传代法开发了一个 F 群体。F 个体和 F RIL 与亲本一起在不同地点进行了测试。使用 90K 单核苷酸多态性（SNP）小麦阵列对 F 个体和双亲进行基因型分析。在染色体 1B（QSrGH.cs-1BL）和 2A（QSrGH.cs-2AL）的长臂上检测到抗秆锈病 QTL。QSrGH.cs-1BL 和 QSrGH.cs-2AL 均由 GH 提供，在 GH/M14 RIL 群体中分别解释了 22%和 18%的成株期抗秆锈病反应变异。与单独携带 QSrGH.cs-1BL 和 QSrGH.cs-2AL 的 RIL 相比，携带这些 QTL 组合的 RIL 减轻了超过 14%的秆锈病严重程度。通过标记基因型分析，证明 QSrGH.cs1BL 与 Sr58/Lr46/Yr29/Pm39 相同。没有 QSrGH.cs-1BL 的系用于 Mendelise QSrGH.cs-2AL。根据先前编目的抗秆锈病基因的基因组位置和 QSrGH.cs-2AL 图谱，它似乎代表了一个新的 APR 基因座，并被永久命名为 Sr63。与 Sr63 相关的 SNP 标记被转化为竞争等位基因特异性 PCR（KASP）检测，并在一组硬粒小麦品种上进行了验证。
