Centre National de Recherche en Génomique Humaine (CNRGH), Institut de Biologie François Jacob, CEA, Université Paris-Saclay, Evry, France.
Sci Data. 2023 Jul 6;10(1):433. doi: 10.1038/s41597-023-02348-z.
Peripheral blood mononuclear cells (PBMCs) are blood cells that are a critical part of the immune system used to fight off infection, defending our bodies from harmful pathogens. In biomedical research, PBMCs are commonly used to study global immune response to disease outbreak and progression, pathogen infections, for vaccine development and a multitude of other clinical applications. Over the past few years, the revolution in single-cell RNA sequencing (scRNA-seq) has enabled an unbiased quantification of gene expression in thousands of individual cells, which provides a more efficient tool to decipher the immune system in human diseases. In this work, we generate scRNA-seq data from human PBMCs at high sequencing depth (>100,000 reads/cell) for more than 30,000 cells, in resting, stimulated, fresh and frozen conditions. The data generated can be used for benchmarking batch correction and data integration methods, and to study the effect of freezing-thawing cycles on the quality of immune cell populations and their transcriptomic profiles.
外周血单核细胞（PBMCs）是血液细胞的重要组成部分，是免疫系统的关键部分，用于抵御感染，保护我们的身体免受有害病原体的侵害。在生物医学研究中，PBMC 通常用于研究对疾病爆发和进展、病原体感染、疫苗开发和许多其他临床应用的全球免疫反应。在过去的几年中，单细胞 RNA 测序（scRNA-seq）的革命使对数千个单个细胞中的基因表达进行无偏定量成为可能，这为破译人类疾病中的免疫系统提供了更有效的工具。在这项工作中，我们在静息、刺激、新鲜和冷冻条件下，对超过 30000 个细胞进行了高测序深度（>100000 个读/cell）的 scRNA-seq 数据生成。生成的数据可用于基准批处理校正和数据集成方法，并研究冷冻-解冻循环对免疫细胞群及其转录组谱质量的影响。
