Khan Shaheen, Kaihara Kelly A
Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Digital Biology Center, Bio-Rad Laboratories, Pleasanton, CA, USA.
Methods Mol Biol. 2019;1979:155-176. doi: 10.1007/978-1-4939-9240-9_10.
Peripheral blood mononuclear cells (PBMCs) are blood cells that are a critical part of the immune system used to fight off infection. However, due to the complexity of PBMCs, which contain multiple different cell types, studying the function of the individual cell types can be difficult, and often studies rely on bulk measurements. Here, we describe the analysis of PBMCs using single-cell RNA-sequencing in droplets. Data from these studies allow for the identification and quantification of the subpopulation of cells that make up the PBMC sample. In addition, differential gene expression between cell types and samples can be assessed.
外周血单个核细胞（PBMCs）是血细胞，是用于抵抗感染的免疫系统的关键组成部分。然而，由于PBMCs的复杂性，其包含多种不同的细胞类型，研究单个细胞类型的功能可能很困难，而且通常研究依赖于整体测量。在这里，我们描述了使用液滴中的单细胞RNA测序对PBMCs进行分析。这些研究的数据允许识别和量化构成PBMC样本的细胞亚群。此外，还可以评估细胞类型和样本之间的差异基因表达。
