Ma Zihang, Guo Xiaoying, Zhang Jun, Jiang Qifeng, Liang Wuying, Meng Wenxin, Chen Shuaijiang, Zhu Yufan, Ye Cundong, Jia Kun
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Guangdong Technological Engineering Research Center for Pet, Guangzhou, China.
Front Vet Sci. 2023 Jun 21;10:1162407. doi: 10.3389/fvets.2023.1162407. eCollection 2023.
Femoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal stem cells (cBMSCs) and gelatin-nano-hydroxyapatite (Gel-nHAP) and evaluate its therapeutic effect on bone defect diseases in dogs. Experiments were performed to evaluate the following: (1) the porosity of Gel-nHAP; (2) the adhesion of cBMSCs to Gel-nHAP; and (3) the effect of Gel-nHAP on cBMSC proliferation. The efficacy and safety of the combination of cBMSC and Gel-nHAP in the repair of femoral shaft defects were evaluated in animal experiments. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In the animal bone defect repair experiment, significant cortical bone growth was observed in the Gel-nHAP group at week 8 ( < 0.05) and in the cBMSCs-Gel-nHAP group at week 4 ( < 0.01). We demonstrated that Gel-nHAP could promote the repair of bone defects, and the effect of cBMSC-Gel-nHAP on the repair of bone defects was profound.
股骨干骨折是犬类常见的骨创伤。间充质干细胞在骨缺损应用中的局限性在于细胞悬液无法固定在骨缺损部位。在本研究中，我们的目的是证实犬骨髓间充质干细胞（cBMSCs）与明胶-纳米羟基磷灰石（Gel-nHAP）的联合应用，并评估其对犬类骨缺损疾病的治疗效果。进行实验以评估以下内容：（1）Gel-nHAP的孔隙率；（2）cBMSCs与Gel-nHAP的粘附性；（3）Gel-nHAP对cBMSC增殖的影响。在动物实验中评估了cBMSC与Gel-nHAP联合修复股骨干缺损的疗效和安全性。结果表明，Gel-nHAP支持cBMSCs的附着，并表现出良好的生物相容性。在动物骨缺损修复实验中，Gel-nHAP组在第8周观察到明显的皮质骨生长（<0.05），cBMSCs-Gel-nHAP组在第4周观察到明显的皮质骨生长（<0.01）。我们证明Gel-nHAP可以促进骨缺损的修复，并且cBMSC-Gel-nHAP对骨缺损修复的效果显著。
