Food Laboratory of Zhongyuan, Flavour Science Research Center of Zhengzhou University, Kexue Avenue 100, Zhengzhou, Henan 450001, P. R. China.
College of Chemistry, Zhengzhou University, Kexue Avenue 100, Zhengzhou, Henan 450001, P. R. China.
Analyst. 2023 Aug 7;148(16):3724-3729. doi: 10.1039/d3an00553d.
An electrochemical glucose sensor based on flexible materials is significant for wearable devices used for real-time health monitoring and diagnosis. However, applying flexible electrodes involves complex fabrication processes and might reduce detection sensitivity. To overcome these obstacles, we herein report a novel strategy for preparing a highly flexible enzyme electrode based on an electrospun poly(vinyl alcohol) (PVA) mat decorated with grown silver nanoparticles (nano-Ag) for electrochemical glucose sensing. Ferrocene (Fc) was selected as an electron acceptor for glucose oxidase (GOD) in order to minimize the influence of oxygen. Electron transfer between GOD and Fc was facilitated by confining them within a mixed self-assembled monolayer (SAM) formed on a thin layer of gold deposited on top of the PVA/nano-Ag film. Nano-Ag was found to significantly increase the surface area of the electrode and improve the stability of electrode conductivity during tensile deformation. Electrochemical glucose detection was performed by chronoamperometry in the electroactivity domain of ferrocene, and good linearity ( = 0.993) was obtained in the range of 0.2-7 mM with a detection limit of 0.038 mM and a relative standard deviation (RSD) of 1.45% ( = 6). After being stuck to a bendable PDMS slice and bent, respectively, at 30° and 60° 50 times, the electrode showed slight changes in detection results (<4.78%), which remained within 8% when the bending angle increased to 90°. With its high flexibility, good detection performance, and convenient fabrication process, the proposed enzyme electrode showed good potential as a flexible platform for wearable glucose sensing systems.
基于柔性材料的电化学生物葡萄糖传感器对于用于实时健康监测和诊断的可穿戴设备具有重要意义。然而，应用柔性电极涉及复杂的制造工艺，并且可能会降低检测灵敏度。为了克服这些障碍，我们在此报告了一种基于电纺聚（聚乙烯醇）（PVA）毡上生长的银纳米粒子（nano-Ag）制备高度灵活的酶电极的新策略，用于电化学生物葡萄糖传感。选择二茂铁（Fc）作为葡萄糖氧化酶（GOD）的电子受体，以最大程度地减少氧气的影响。通过将它们限制在金薄层上形成的混合自组装单层（SAM）内，促进了 GOD 和 Fc 之间的电子转移，该金薄层沉积在 PVA/nano-Ag 薄膜的顶部。发现 nano-Ag 可显著增加电极的表面积，并提高电极在拉伸变形过程中的导电性稳定性。通过计时安培法在 ferrocene 的电活性范围内进行葡萄糖电化学检测，在 0.2-7 mM 的范围内获得了良好的线性（ = 0.993），检测限为 0.038 mM，相对标准偏差（RSD）为 1.45％（ = 6）。将电极分别粘贴到可弯曲的 PDMS 薄片上，并分别以 30°和 60°弯曲 50 次后，检测结果的变化很小（<4.78％），当弯曲角度增加到 90°时，变化仍保持在 8％以内。该酶电极具有高柔韧性，良好的检测性能和简便的制造工艺，显示出作为用于可穿戴葡萄糖感测系统的柔性平台的良好潜力。
