Departamento de Farmacobiología, Universidad de Guadalajara, Centro Universitario de Ciencias Exactas E Ingenierías (CUCEI), Blvd. Marcelino García Barragán 1421, CP 44430, Guadalajara, Jalisco, México.
Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur , La Paz, BCS, 23096, México.
Vet Res Commun. 2023 Dec;47(4):2041-2053. doi: 10.1007/s11259-023-10156-4. Epub 2023 Jul 7.
Moringa oleifera is one of the most promising plants in aquaculture because it improves the health status, zootechnical parameters and resistance against diseases. This research evaluates the physicochemical, antioxidant values of spray-dried Moringa oleifera seed extract microencapsulates obtained at 140 and 180 °C with whey protein concentrate (WPC) and maltodextrin (MD) as wall materials in two different proportions: WPC 100% and WPC-MD (3:1). Also, immune response of peripheral blood leukocytes (PBL) of Longfin yellowtail Seriola rivoliana stimulated with spray-dried Moringa oleifera seed for 24 h was assessed. The physicochemical parameters show that the recovery yield for all the treatments was of 65% and microencapsulates demonstrated to be stable in the physicochemical tests with low solubilization times and protection against humidity. For WPC-MD (3:1)/140 °C, bioactive compound retention and antioxidant potential were higher than in other combinations. The immunological test show that any treatments was non-cytotoxic against peripheral blood leukocytes. WPC-MD (3:1)/140 °C treatment enhanced immune parameters as phagocytosis, respiratory burst, myeloperoxidase activities and nitric oxide production. Immune related genes as IL-1β and TNF-α were up-regulated in those stimulated leukocytes with WPC-MD (3:1)/140 °C. The results suggest that this combination may be a good alternative for animal health as a medicinal and immunostimulant additive.
辣木是水产养殖中最有前途的植物之一，因为它可以改善健康状况、动物生产性能和疾病抵抗力。本研究评估了喷雾干燥辣木种子提取物微胶囊的物理化学性质和抗氧化值，这些微胶囊是在 140 和 180°C 下用乳清蛋白浓缩物（WPC）和麦芽糊精（MD）作为壁材制成的，其比例为 WPC100%和 WPC-MD（3:1）两种。此外，还评估了用喷雾干燥辣木种子刺激长鳍黄尾鲷外周血白细胞（PBL）24 小时后的免疫反应。物理化学参数表明，所有处理的回收产率均为 65%，微胶囊在物理化学测试中表现稳定，具有较低的溶解度和防潮保护。对于 WPC-MD（3:1）/140°C，生物活性化合物保留率和抗氧化能力均高于其他组合。免疫试验表明，任何处理对外周血白细胞均无细胞毒性。WPC-MD（3:1）/140°C 处理增强了吞噬作用、呼吸爆发、髓过氧化物酶活性和一氧化氮产生等免疫参数。在那些用 WPC-MD（3:1）/140°C 刺激的白细胞中，IL-1β和 TNF-α等免疫相关基因上调。结果表明，这种组合可能是一种很好的替代物，可作为一种药用和免疫刺激添加剂，用于动物健康。
