Maeno Hinata, Ogata Satoshi, Shimizu Tetsuhide, Yang Ming
Department of Mechanical System Engineering, Faculty of System Design, Tokyo Metropolitan University, Tokyo 191-0065, Japan.
Micromachines (Basel). 2023 Mar 28;14(4):744. doi: 10.3390/mi14040744.
A film-stacked structure consisting of polyethylene terephthalate (PET) films stacked in a gap of 20 µm that can be combined with 96-well microplates used in biochemical analysis has been developed by the authors. When this structure is inserted into a well and rotated, convection flow is generated in the narrow gaps between the films to enhance the chemical/bio reaction between the molecules. However, since the main component of the flow is a swirling flow, only a part of the solution circulates into the gaps, and reaction efficiency is not achieved as designed. In this study, an unsteady rotation is applied to promote the analyte transport into the gaps using the secondary flow generated on the surface of the rotating disk. Finite element analysis is used to evaluate the changes in flow and concentration distribution for each rotation operation and to optimize the rotation conditions. In addition, the molecular binding ratio for each rotation condition is evaluated. It is shown that the unsteady rotation accelerates the binding reaction of proteins in an ELISA (Enzyme Linked Immunosorbent Assay), a type of immunoassay.
作者开发了一种由聚对苯二甲酸乙二酯（PET）薄膜堆叠而成的薄膜堆叠结构，这些薄膜以20微米的间隙堆叠，可与生化分析中使用的96孔微孔板相结合。当将这种结构插入孔中并旋转时，会在薄膜之间的狭窄间隙中产生对流，以增强分子之间的化学/生物反应。然而，由于流动的主要成分是旋流，只有一部分溶液循环到间隙中，反应效率未达到设计要求。在本研究中，通过施加非定常旋转，利用旋转盘表面产生的二次流促进分析物传输到间隙中。使用有限元分析来评估每次旋转操作时的流动和浓度分布变化，并优化旋转条件。此外，还评估了每种旋转条件下的分子结合率。结果表明，非定常旋转加速了酶联免疫吸附测定（ELISA）（一种免疫测定类型）中蛋白质的结合反应。
