Liu Xiaoying, Wang Xueqing, Yang Chenxiao, Wang Guangyu, Fan Baoli, Shang Yuntao, Dang Chen, Xie Chaojie, Wang Zhenying
College of Life Science, Tianjin Normal University, Tianjin, 30087, China.
Tianjin Key Laboratory of Water Resources and Environment, Tianjin Normal University, Tianjin, 30087, China.
Int J Biol Macromol. 2023 Sep 1;248:125691. doi: 10.1016/j.ijbiomac.2023.125691. Epub 2023 Jul 6.
Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting wheat crops worldwide. Functional genes can be activated in response to Bgt inoculations. Calcineurin B-like protein (CBL) together with CBL-interacting protein kinase (CIPK) forms the CBL-CIPK protein complex that participates in Ca sensor kinase-related signaling pathways responding to abiotic and biotic stresses. In this study, we performed a genome-wide screening and identified 27 CIPK subfamilies (123 CIPK transcripts, TaCIPKs) including 55 new and 47 updated TaCIPKs in wheat. Phylogenetic analysis revealed that 123 TaCIPKs could be divided into four groups. Segmental duplications and tandem repeats promoted the expansion of the TaCIPK family. Gene function was further evidenced by differences in gene structure, cis-elements, and protein domains. TaCIPK15-4A was cloned in this study. TaCIPK15-4A contained 17 serine, seven tyrosine, and 15 threonine phosphorylation sites and localized in the plasma membrane and cytoplasm. TaCIPK15-4A expression was induced after Bgt inoculation. Virus-induced gene silencing and overexpression experiments indicated that TaCIPK15-4A could play a positive role in wheat disease resistance to Bgt. Overall, these results provide insights into the role of the TaCIPK gene family in wheat resistance and could be beneficial for further research to prevent Bgt infection.
由小麦白粉病菌（Blumeria graminis f. sp. tritici，Bgt）引起的白粉病是一种影响全球小麦作物的毁灭性病害。功能基因可响应Bgt接种而被激活。类钙调神经磷酸酶B蛋白（CBL）与CBL互作蛋白激酶（CIPK）共同形成CBL-CIPK蛋白复合体，参与响应非生物和生物胁迫的钙传感器激酶相关信号通路。在本研究中，我们进行了全基因组筛选，在小麦中鉴定出27个CIPK亚家族（123个CIPK转录本，TaCIPKs），其中包括55个新的和47个更新的TaCIPKs。系统发育分析表明，123个TaCIPKs可分为四组。片段重复和串联重复促进了TaCIPK家族的扩张。基因结构、顺式元件和蛋白质结构域的差异进一步证明了基因功能。本研究克隆了TaCIPK15-4A。TaCIPK15-4A含有17个丝氨酸、7个酪氨酸和15个苏氨酸磷酸化位点，定位于质膜和细胞质中。Bgt接种后TaCIPK15-4A的表达被诱导。病毒诱导的基因沉默和过表达实验表明，TaCIPK15-4A在小麦对Bgt的抗病性中可发挥积极作用。总体而言，这些结果为TaCIPK基因家族在小麦抗性中的作用提供了见解，可能有助于进一步开展预防Bgt感染的研究。
