Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China.
Tianjin Key Laboratory of Water Resources and Environment, Tianjin Normal University, Tianjin 300387, China.
Int J Mol Sci. 2024 Jun 15;25(12):6603. doi: 10.3390/ijms25126603.
Wheat powdery mildew is an important fungal disease that seriously jeopardizes wheat production, which poses a serious threat to food safety. SJ106 is a high-quality, disease-resistant spring wheat variety; this disease resistance is derived from Wheat-wheatgrass 33. In this study, the powdery mildew resistance genes in SJ106 were located at the end of chromosome 6DS, a new disease resistance locus tentatively named . This interval was composed of a nucleotide-binding leucine-rich repeat (NLR) gene cluster containing 19 NLR genes. Five NLRs were tandem duplicated genes, and one of them (a coiled coil domain-nucleotide binding site-leucine-rich repeat (CC-NBS-LRR; CNL) type gene, ) expressed 69-836-fold in SJ106 compared with the susceptible control. The genome DNA and cDNA sequences of TaRGA5-like were amplified from SJ106, which contain several nucleotide polymorphisms in LRR regions compared with susceptible individuals and Chinese Spring. Overexpression of significantly increased resistance to powdery mildew in susceptible receptor wheat Jinqiang5. However, Virus induced gene silence (VIGS) of resulted in only a small decrease of SJ106 in disease resistance, presumably compensated by other duplicated genes. The results suggested that confers partial powdery mildew resistance in SJ106. As a member of the , functioned together with other duplicated genes to improve wheat resistance to powdery mildew. Wheat variety SJ106 would become a novel and potentially valuable germplasm for powdery mildew resistance.
小麦白粉病是一种重要的真菌病害，严重威胁小麦生产，对食品安全构成严重威胁。SJ106 是一种优质、抗病的春小麦品种；这种抗病性来自于小麦-冰草 33。在这项研究中，SJ106 中的白粉病抗性基因位于 6DS 染色体的末端，一个新的抗病基因位点暂命名为 。该区间由一个核苷酸结合富含亮氨酸重复（NLR）基因簇组成，包含 19 个 NLR 基因。五个 NLR 是串联重复基因，其中一个（卷曲螺旋结构域-核苷酸结合位点-富含亮氨酸重复（CC-NBS-LRR；CNL）类型基因，）在 SJ106 中的表达量比感病对照高 69-836 倍。从 SJ106 中扩增出 TaRGA5-like 的基因组 DNA 和 cDNA 序列，与感病个体和中国春相比，LRR 区域存在几个核苷酸多态性。在易感受体小麦金强 5 中过表达 显著提高了对白粉病的抗性。然而， 沉默（VIGS）仅导致 SJ106 抗病性略有下降，可能由其他 基因的重复基因补偿。结果表明 赋予 SJ106 部分白粉病抗性。作为 的一员， 与其他 基因的重复共同作用，提高了小麦对白粉病的抗性。小麦品种 SJ106 将成为一种新的、有潜在价值的抗白粉病种质资源。
