College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China; Centre for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, 117574 Singapore, Singapore.
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China.
Int J Biol Macromol. 2023 Sep 1;248:125729. doi: 10.1016/j.ijbiomac.2023.125729. Epub 2023 Jul 7.
Efficient enzyme immobilization systems offer a promising approach for improving enzyme stability and recyclability, reducing enzyme contamination in products, and expanding the applications of enzymes in the biomedical field. Covalent organic frameworks (COFs) possess high surface areas, ordered channels, optional building blocks, highly tunable porosity, stable mechanical properties, and abundant functional groups, making them ideal candidates for enzyme immobilization. Various COF-enzyme composites have been successfully synthesized, with performances that surpass those of free enzymes in numerous ways. This review aims to provide an overview of current enzyme immobilization strategies using COFs, highlighting the characteristics of each method and recent research applications. The future opportunities and challenges of enzyme immobilization technology using COFs are also discussed.
高效的酶固定化体系为提高酶的稳定性和可重复使用性、减少酶在产品中的污染以及拓展酶在生物医学领域的应用提供了一种很有前途的方法。共价有机框架（COFs）具有高比表面积、有序孔道、可选的构筑单元、高度可调的孔隙率、稳定的机械性能和丰富的功能基团，使其成为酶固定化的理想候选材料。已经成功合成了各种 COF-酶复合材料，其性能在许多方面都超过了游离酶。本综述旨在概述当前使用 COFs 的酶固定化策略，重点介绍每种方法的特点和最近的研究应用。还讨论了 COFs 用于酶固定化技术的未来机遇和挑战。
