Zhang Jin, Li You, Li Jiaxuan, Zhu Lin, Jin Shangbin
State Key Laboratory of Fluorine & Nitrogen Chemicals, School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, China.
Nat Commun. 2025 Jul 1;16(1):6046. doi: 10.1038/s41467-025-60719-z.
In nature, enzymes in biofilms often lack robustness and chemical functionality. Covalent organic frameworks (COFs) have emerged as a robust platform for enzyme immobilization, but mimicking biofilm structures for in-situ enzyme immobilization remains challenging. Here, we report a biomimetic strategy to synthesize COFs with various morphologies under mild conditions using Gemini surfactants. These surfactants mimic phospholipid bilayers, enabling in-situ enzyme immobilization. Controlled COFs morphologies (vesicular and lamellar) are formed by cationic Gemini surfactants through micelle or reverse micelle regulation. Enzymes with different charges induce distinct COFs morphologies, which in turn affect enzyme activity. This work offers insights into biomimetic morphology control of COFs and their application in enzyme immobilization.
在自然界中，生物膜中的酶通常缺乏稳定性和化学功能。共价有机框架（COF）已成为一种用于固定酶的稳定平台，但模仿生物膜结构进行原位酶固定仍然具有挑战性。在此，我们报道了一种仿生策略，即在温和条件下使用双子表面活性剂合成具有各种形态的COF。这些表面活性剂模仿磷脂双层，能够实现原位酶固定。阳离子双子表面活性剂通过胶束或反胶束调控形成可控的COF形态（囊泡状和层状）。带不同电荷的酶会诱导出不同的COF形态，进而影响酶的活性。这项工作为COF的仿生形态控制及其在酶固定中的应用提供了见解。
