Enzymatic Conversion of Mixed Neem () and Nile Tilapia () Oils into Biolubricants: A Green Biocatalytic Approach.

This study focused on optimizing the enzymatic synthesis of biolubricants using a 20% blend of Nile tilapia () and neem () oils catalyzed by Eversa Transform 2.0. The optimal conditionsthe molar ratio of 1:5 (mol/mol), 10% biocatalyst load, and a reaction time of 48 hachieved a predicted ester conversion of 97%, with experimental validation confirming 92.80 ± 0.03%. Molecular docking demonstrated stable complexes, particularly with decanoic and linoleic acids, showing Root Mean Square Deviation (RMSD) values below 2.0 Å, indicating minimal conformational changes. Gas chromatography and mass spectrometry (GC-MS) analysis identified key esters like 2-ethylhexyl and octyl, which enhance thermal stability, lubricity, and oxidation resistance. Fourier Transform Infrared Spectroscopy (FT-IR) analysis confirmed effective ester formation, corroborating experimental results. Viscosity (2.4511 mm/s) and density (0.84259 g/cm) values aligned with ISO VG 3 (International Organization for Standardization Viscosity Grade) standards, suitable for low-load and low-speed applications.