Liu Ru, Xi Peng, Yang Ning, Luo Ying, Cheng Bowen
School of Materials Science and Engineering, Tiangong University, Tianjin 300387, PR China.
State Key Laboratory of Separation Membranes & Membrane Process, Tiangong University, Tianjin 300387, PR China; School of Materials Science and Engineering, Tiangong University, Tianjin 300387, PR China; Tianjin Key Laboratory of Advanced Fibers and Energy Storage, Tianjin 300387, PR China.
Int J Biol Macromol. 2023 Aug 15;246:125731. doi: 10.1016/j.ijbiomac.2023.125731. Epub 2023 Jul 6.
An ideal wound dressing can absorb wound exudate in time, and has the advantages of moisture permeability, oxygen permeability, rapid hemostatic performance, antibacterial and low-toxic, which are the key to wound healing. However, traditional wound dressings exist structural and functional defects, especially in controlling bleeding and active wound protection. Herein, a novel three-dimensional chitosan/ poly (ethylene oxide) sponge dressing (3D CS/PEO sponge-ZPC) consists of CS/PEO nanofiber sponge (carrier unit), Zn metal-organic framework grown in-situ (Zn-MOF, drug loading unit and antibacterial unit), curcumin (CUR, antibacterial unit), and poly[(N-isopropylacrylamide)-co-(methacrylic acid)] (P(NIPAM-co-MAA), 'gatekeepers' unit) to promote the wound healing by absorb exudate in time, accelerate hemostasis and inhibit bacteria growth. Due to the unique structure of the as-prepared 3D CS/PEO sponge-ZPC was endowed with smart stimuli-responsive drug release mode, rapid hemostatic performance and strong antibacterial property. The result of CUR release showed smart "ON-OFF" drug release mode. Antibacterial results verified strong antibacterial property up to 99.9 %. Hemolysis test showed that hemolysis ratio of 3D CS/PEO sponge-ZPC met the acceptable standard. The rapid hemostatic property was demonstrated by hemostatic test. High wound healing effect was confirmed in vivo. These results provide an important research basis for the design of new smart dressing.
理想的伤口敷料能够及时吸收伤口渗出液，具有透湿性、透气性、快速止血性能、抗菌且低毒等优点，这些是伤口愈合的关键。然而，传统伤口敷料存在结构和功能缺陷，尤其是在控制出血和主动保护伤口方面。在此，一种新型的三维壳聚糖/聚环氧乙烷海绵敷料（3D CS/PEO海绵-ZPC）由CS/PEO纳米纤维海绵（载体单元）、原位生长的锌金属有机框架（Zn-MOF，载药单元和抗菌单元）、姜黄素（CUR，抗菌单元）以及聚（N-异丙基丙烯酰胺）-co-（甲基丙烯酸）（P（NIPAM-co-MAA），“守门人”单元）组成，通过及时吸收渗出液、加速止血和抑制细菌生长来促进伤口愈合。由于所制备的3D CS/PEO海绵-ZPC的独特结构，其具有智能刺激响应药物释放模式、快速止血性能和强大的抗菌性能。CUR释放结果显示出智能的“开-关”药物释放模式。抗菌结果证实其抗菌性能高达99.9%。溶血试验表明3D CS/PEO海绵-ZPC的溶血率符合可接受标准。止血试验证明了其快速止血性能。体内实验证实了其具有高伤口愈合效果。这些结果为新型智能敷料的设计提供了重要的研究依据。
