College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China.
Research and Development Center for Watershed Environmental Eco-Engineering, Beijing Normal University, Zhuhai, China.
J Appl Toxicol. 2023 Dec;43(12):1806-1818. doi: 10.1002/jat.4514. Epub 2023 Jul 9.
Bisphenol AF (BPAF) is one of the most commonly used alternatives of bisphenol A in the plastics industry. The effects of BPAF on nervous development are unclear. Curcumin (CUR) has been determined to be an anti-inflammatory and antioxidant agent. In this study, the effects of BPAF on neurotoxicity of zebrafish embryos/larvae and whether CUR could reverse effects induced by BPAF were investigated. The results showed that BPAF treatment induced deficits in locomotor behavior, altered the larval brain development, caused aberrant expression of neurogenesis related genes (elavl3, zn5, α-tubulin, syn2a, and gap43), decreased acetylcholinesterase (AChE) activity, and induced oxidative stress, cell apoptosis, and neuroinflammation in zebrafish larvae. CUR addition could block the adverse effects of BPAF on nervous development by attenuated oxidative stress and cell apoptosis induced by BPAF in zebrafish, enhanced the activity of AChE, and increased the expression of genes involved in the pro-inflammatory cytokines (IL-6, IL-1β, TNF-α, and IL-8). The results of this study indicate that BPAF could induce aberrant development on nervous system. However, CUR exerts neuroprotective effects on BPAF-induced neurotoxicity in zebrafish larvae.
双酚 AF（BPAF）是塑料工业中双酚 A 的最常用替代品之一。BPAF 对神经发育的影响尚不清楚。姜黄素（CUR）已被确定为一种抗炎和抗氧化剂。在这项研究中，研究了 BPAF 对斑马鱼胚胎/幼虫神经毒性的影响，以及 CUR 是否可以逆转 BPAF 引起的作用。结果表明，BPAF 处理诱导运动行为缺陷，改变幼虫大脑发育，引起神经发生相关基因（elavl3、zn5、α-微管蛋白、syn2a 和 gap43）的异常表达，降低乙酰胆碱酯酶（AChE）活性，并诱导氧化应激、细胞凋亡和神经炎症在斑马鱼幼虫中。CUR 的添加可以通过减轻 BPAF 诱导的氧化应激和细胞凋亡来阻断 BPAF 对神经发育的不良影响，增强 AChE 的活性，并增加参与促炎细胞因子（IL-6、IL-1β、TNF-α 和 IL-8）的基因表达。这项研究的结果表明，BPAF 可诱导神经系统发育异常。然而，CUR 对 BPAF 诱导的斑马鱼幼虫神经毒性具有神经保护作用。
