Li Rongzhen, Liu Shuai, Qiu Wenhui, Yang Feng, Zheng Yi, Xiong Ying, Li Guanrong, Zheng Chunmiao
State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
Environ Sci Ecotechnol. 2020 Aug 5;4:100054. doi: 10.1016/j.ese.2020.100054. eCollection 2020 Oct.
Bisphenol AF (BPAF), an alternative to bisphenol A, is widely detected in aquatic environments. Owing to health concerns, the toxic effects of BPAF on organisms are drawing attention. The present study aims to evaluate the toxicity of BPAF, combining the results of omics techniques and experiment. Employing transcriptome sequencing (RNA-seq), we obtained 391, 648, 512, and 545 differentially expressed genes (DEGs) in 0.1, 1, 10, and 100 μg/L BPAF-exposed zebrafish larvae, respectively. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed the early development, stimulus-response, and MAPK signaling pathway were significantly affected by BPAF. In addition, five hub genes (, , , , and ) were highlighted as the key genes in MAPK signaling pathway using the protein-protein interaction network. Therefore, the RNA-seq results showed that early development and stimulus-response were the main processes affected by BPAF, which was consistent with our morphological and pathological results. The hatching rate of zebrafish embryos in 1 and 10 μg/L BPAF groups was significantly inhibited, and the oxidative stress indexes, including the level of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and lipid peroxidation (LPO), were significantly increased by the 100 μg/L BPAF treatment. Moreover, the activity of alkaline phosphatase (AKP) was significantly decreased in all BPAF exposure groups. In conclusion, exposure to BPAF at environmental relevant concentrations affected the early development and immune system of zebrafish larvae by modulating MAPK signaling pathway, and our results provide solid evidence for the future studies on the toxicity of bisphenols.
双酚AF（BPAF）作为双酚A的替代品，在水生环境中被广泛检测到。出于对健康的担忧，BPAF对生物体的毒性作用正受到关注。本研究旨在结合组学技术和实验结果来评估BPAF的毒性。通过转录组测序（RNA-seq），我们分别在暴露于0.1、1、10和100μg/L BPAF的斑马鱼幼体中获得了391、648、512和545个差异表达基因（DEG）。基因本体（GO）分析和京都基因与基因组百科全书（KEGG）富集显示，早期发育、刺激反应和丝裂原活化蛋白激酶（MAPK）信号通路受到BPAF的显著影响。此外，利用蛋白质-蛋白质相互作用网络，五个枢纽基因（，，，，和）被确定为MAPK信号通路中的关键基因。因此，RNA-seq结果表明，早期发育和刺激反应是受BPAF影响的主要过程，这与我们的形态学和病理学结果一致。1和10μg/L BPAF组斑马鱼胚胎的孵化率受到显著抑制，100μg/L BPAF处理显著提高了包括总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）和脂质过氧化（LPO）水平在内的氧化应激指标。此外，所有BPAF暴露组的碱性磷酸酶（AKP）活性均显著降低。总之，在环境相关浓度下暴露于BPAF会通过调节MAPK信号通路影响斑马鱼幼体的早期发育和免疫系统，我们的结果为未来双酚类毒性研究提供了确凿证据。
