Department of Land, Environment, Agriculture and Forestry (TESAF), University of Padova, Viale dell'Università 16, 35020 Legnaro, PD, Italy.
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via S. Camillo de Lellis snc, 01100 Viterbo, VT, Italy.
Int J Mol Sci. 2021 Oct 6;22(19):10811. doi: 10.3390/ijms221910811.
Fungal enzymes degrading the plant cell wall, such as xylanases, can activate plant immune responses. The FGSG_03624 xylanase, previously shown to elicit necrosis and hydrogen peroxide accumulation in wheat, was investigated for its ability to induce disease resistance. To this aim, we transiently and constitutively expressed an enzymatically inactive form of FGSG_03624 in tobacco and , respectively. The plants were challenged with pv. or pv. and . Symptom reduction by the bacterium was evident, while no reduction was observed after inoculation. Compared to the control, the presence of the xylanase gene in transgenic plants did not alter the basal expression of a set of defense-related genes, and, after the inoculation, a prolonged PR1 expression was detected. inoculation experiments of durum wheat spikes exogenously treated with the FGSG_03624 xylanase highlighted a reduction of symptoms in the early phases of infection and a lower fungal biomass accumulation than in the control. Besides, callose deposition was detected in infected spikes previously treated with the xylanase and not in infected control plants. In conclusion, our results highlight the ability of FGSG_03624 to enhance plant immunity, thus decreasing disease severity.
真菌细胞壁降解酶，如木聚糖酶，可以激活植物的免疫反应。先前研究表明，FGSG_03624 木聚糖酶能引起小麦坏死和过氧化氢积累，我们研究了它诱导抗病性的能力。为此，我们分别在烟草和 中瞬时和组成型表达了一种酶失活形式的 FGSG_03624。用 pv. 或 pv. 对植物进行了挑战。细菌引起的症状减轻明显，而接种 后则没有观察到症状减轻。与对照相比，木聚糖酶基因在转基因 植物中的存在并未改变一组防御相关基因的基础表达，并且在 接种后，检测到 PR1 表达延长。用 FGSG_03624 木聚糖酶外源处理冬小麦穗进行接种实验，结果表明，在感染早期，感染症状减轻，真菌生物量积累低于对照。此外，在先前用木聚糖酶处理的感染穗中检测到了几丁质的沉积，而在感染的对照植物中则没有检测到。总之，我们的结果强调了 FGSG_03624 增强植物免疫力的能力，从而降低了疾病的严重程度。
