用于猪、绵羊和牛中表面免疫球蛋白（sIg）阳性淋巴细胞检测的直接抗球蛋白玫瑰花结反应（DARR）与直接免疫荧光法（DIF）的比较
Comparison of the direct antiglobulin rosetting reaction (DARR) and direct immunofluorescence (DIF) for demonstration of sIg-bearing lymphocytes in pigs, sheep and cattle.
作者信息
Binns R M, Licence S T, Symons D B, Gurner B W, Coombs R R, Walters D E
出版信息
Immunology. 1979 Mar;36(3):549-55.
Tests with untreated and trypsin-treated red cells (rbc) from a variety of species showed that anti-Ig-coupled pig RBC are good indicator cells for the study of ruminant blood sIg + lymphocytes by the DARR test; coupled donkey and rabbit RBC are suitable for investigating pig lymphocytes. The different species showed the following percentages of sIg + lymphocytes (M +/- SE) by direct immunofluorescence (DIF) and the direct antiglobulin rosetting reaction (DARR) respectively:pigs 9.2 +/- 0.7% and 16.3 +/- 1.2%; sheep 20.2 +/- 1.2% and 33.1 +/- 1.6%; Cattle 13.5 +/- 1.4% and 28.9 +/- 3.5%. The mean ratio of sIg + lymphocytes shown by the two tests (DARR/DIF) for each species was 1.80 +/- 0.08 for pigs, 1.73 +/- 0.7 for sheep and 2.15 +/- 0.18 for cattle. Preincubation of pig and sheep lymphocytes at 37 degrees for 1 h did not alter the proportion of sIg + lymphocytes detected by either test. Thus the DARR test reveals a further population of sIg + lymphocytes in addition to that detected by immunofluorescence, whose number is proportional to the B population as measured by DIF and whose sIg is intimately associated with the membrane.
对来自多种物种的未处理和经胰蛋白酶处理的红细胞（RBC）进行的测试表明，抗Ig偶联的猪红细胞是通过直接抗球蛋白玫瑰花结反应（DARR）研究反刍动物血液sIg⁺淋巴细胞的良好指示细胞；偶联的驴和兔红细胞适用于研究猪淋巴细胞。不同物种通过直接免疫荧光（DIF）和直接抗球蛋白玫瑰花结反应（DARR）分别显示出以下百分比的sIg⁺淋巴细胞（M±SE）：猪9.2±0.7%和16.3±1.2%；绵羊20.2±1.2%和33.1±1.6%；牛13.5±1.4%和28.9±3.5%。每种物种的两种测试（DARR/DIF）显示的sIg⁺淋巴细胞的平均比例为：猪1.80±0.08，绵羊1.73±0.7，牛2.15±0.18。将猪和绵羊淋巴细胞在37℃预孵育1小时不会改变通过任何一种测试检测到的sIg⁺淋巴细胞的比例。因此，除了通过免疫荧光检测到的sIg⁺淋巴细胞群体外，DARR测试还揭示了另一群体的sIg⁺淋巴细胞，其数量与通过DIF测量的B细胞群体成比例，并且其sIg与细胞膜紧密相关。
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