Guo Yawen, Wang Hairui, Liu Zhaoyu, Chang Zhihui
Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, China.
Front Microbiol. 2023 Jun 23;14:1211835. doi: 10.3389/fmicb.2023.1211835. eCollection 2023.
Pyogenic liver abscess (PLA) patients combined with diabetes mellitus (DM) tend to have more severe clinical manifestations than without DM. The mechanism responsible for this phenomenon is not entirely clear. The current study therefore aimed to comprehensively analyze the microbiome composition and metabolome in pus from PLA patients with and without DM, to determine the potential reasons for these differences.
Clinical data from 290 PLA patients were collected retrospectively. We analyzed the pus microbiota using 16S rDNA sequencing in 62 PLA patients. In addition, the pus metabolomes of 38 pus samples were characterized by untargeted metabolomics analysis. Correlation analyses of microbiota, metabolites and laboratory findings were performed to identify significant associations.
PLA patients with DM had more severe clinical manifestations than PLA patients without DM. There were 17 discriminating genera between the two groups at the genus level, among which was the most discriminating taxa. The ABC transporters was the most significant differential metabolic pathway predicted by PICRUSt2. Untargeted metabolomics analysis showed that concentrations of various metabolites were significantly different between the two groups and seven metabolites were enriched in the ABC transporters pathway. Phosphoric acid, taurine, and orthophosphate in the ABC transporters pathway were negatively correlated with the relative abundance of and the blood glucose level.
The results showed that the relative abundance of in the pus cavity of PLA patients with DM was higher than those without DM, accompanied by changes of various metabolites and metabolic pathways, which may be associated with more severe clinical manifestations.
与非糖尿病患者相比，患有糖尿病（DM）的化脓性肝脓肿（PLA）患者往往有更严重的临床表现。造成这种现象的机制尚不完全清楚。因此，本研究旨在全面分析有或无糖尿病的PLA患者脓液中的微生物组组成和代谢组，以确定这些差异的潜在原因。
回顾性收集290例PLA患者的临床资料。我们对62例PLA患者的脓液微生物群进行了16S rDNA测序分析。此外，通过非靶向代谢组学分析对38份脓液样本的脓液代谢组进行了表征。对微生物群、代谢物和实验室检查结果进行相关性分析，以确定显著关联。
患有糖尿病的PLA患者比无糖尿病的PLA患者有更严重的临床表现。两组在属水平上有17个鉴别属，其中 是最具鉴别力的分类群。ABC转运蛋白是PICRUSt2预测的最显著的差异代谢途径。非靶向代谢组学分析表明，两组间各种代谢物浓度存在显著差异，且有7种代谢物在ABC转运蛋白途径中富集。ABC转运蛋白途径中的磷酸、牛磺酸和正磷酸盐与 的相对丰度和血糖水平呈负相关。
结果表明，患有糖尿病的PLA患者脓腔中 的相对丰度高于无糖尿病患者，同时伴有各种代谢物和代谢途径的变化，这可能与更严重的临床表现有关。
