Choudhary Nisha, Tandi Dipty, Verma Rakesh Kumar, Yadav Virendra Kumar, Dhingra Naveen, Ghosh Tathagata, Choudhary Mahima, Gaur Rajarshi K, Abdellatif Magda H, Gacem Amel, Eltayeb Lienda Bashier, Alqahtani Mohammed S, Yadav Krishna Kumar, Jeon Byong-Hun
Department of Biosciences, School of Liberal Arts and Sciences, Mody University of Science and Technology, Lakshmangarh, Rajasthan, India.
Department of Agriculture, Medi-Caps University, Indore, Madhya Pradesh, India.
Front Plant Sci. 2023 Jun 23;14:1164461. doi: 10.3389/fpls.2023.1164461. eCollection 2023.
The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein-editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein-based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.
通过发现被称为抗CRISPR（Acr）的CRISPR-Cas系统的蛋白质抑制剂，精确且可控的CRISPR-Cas工具得以开发。Acr蛋白能够控制脱靶突变并阻碍Cas蛋白的编辑操作。Acr有助于选择性育种，这可能有助于植物和动物改善其有价值的特征。在本综述中，讨论了几种Acr所采用的基于Acr蛋白的抑制机制，例如（a）CRISPR-Cas复合物组装的中断，（b）对靶DNA结合的干扰，（c）靶DNA/RNA切割的阻断，以及（d）信号分子的酶促修饰或降解。此外，本综述强调了Acr蛋白在植物研究中的应用。
