Peng Xinyan, Jiang Yujun
Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China.
Inflammation. 2014 Oct;37(5):1599-607. doi: 10.1007/s10753-014-9886-1.
This study investigated the protective effect of Lactobacillus plantarum NDC 75017 (L. plantarum NDC 75017) against acute liver injury induced by lipopolysaccharide (LPS). Thirty male mice were randomly divided into the control, LPS, and LPS + L. plantarum NDC 75017 groups. In the LPS + L. plantarum group, the mice were orally pretreated with L. plantarum NDC 75017 for 15 days. At 16 days, the mice in the LPS and LPS + L. plantarum NDC 75017 groups were intraperitoneally injected with LPS at 4 mg/kg body weight, whereas the control mice were treated with an equal amount of saline. After 8 h, the serum alanine transaminase (ALT), aspartate aminotransferase (AST), and histology changes were examined. The oxidative stress markers and pro-inflammatory cytokines in the liver were also examined. Meanwhile, the expression of nuclear factor κB (NF-κB) mRNA and toll-like receptor 4 (TLR4) in the liver was determined by qRT-PCR. The LPS group showed an increase in ALT and AST, whereas the LPS + L. plantarum NDC 75017 group showed a significant decrease. In addition, pretreatment with L. plantarum NDC 75017 can attenuate LPS-induced oxidative stress and inflammatory response. Furthermore, the increase of hepatic NF-κB and TLR4 mRNA induced by LPS was significantly downregulated by the pretreatment with L. plantarum NDC 75017. These data show that pretreatment with L. plantarum NDC 75017 protects against LPS-induced oxidative stress and inflammatory injury in the liver of mice, which may be attributed to the inhibition of the TLR4-NF-κB pathway.
本研究调查了植物乳杆菌NDC 75017（L. plantarum NDC 75017）对脂多糖（LPS）诱导的急性肝损伤的保护作用。30只雄性小鼠被随机分为对照组、LPS组和LPS + 植物乳杆菌NDC 75017组。在LPS + 植物乳杆菌组中，小鼠口服植物乳杆菌NDC 75017预处理15天。在第16天，LPS组和LPS + 植物乳杆菌NDC 75017组的小鼠腹腔注射4 mg/kg体重的LPS，而对照小鼠用等量的生理盐水处理。8小时后，检测血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和组织学变化。还检测了肝脏中的氧化应激标志物和促炎细胞因子。同时，通过qRT-PCR测定肝脏中核因子κB（NF-κB）mRNA和Toll样受体4（TLR4）的表达。LPS组的ALT和AST升高，而LPS + 植物乳杆菌NDC 75017组显著降低。此外，植物乳杆菌NDC 75017预处理可减轻LPS诱导的氧化应激和炎症反应。此外，植物乳杆菌NDC 75017预处理可显著下调LPS诱导的肝脏NF-κB和TLR4 mRNA的增加。这些数据表明，植物乳杆菌NDC 75017预处理可保护小鼠肝脏免受LPS诱导的氧化应激和炎症损伤，这可能归因于对TLR4-NF-κB途径的抑制。
