Freier C, Alberici G, Turk P, Baud F, Bohuon C
Clin Chem. 1986 Sep;32(9):1742-5.
We describe a 3H-based RIA for the antimalarial drug chloroquine (CLQ), the most commonly used antimalarial drug. In the assay a monoclonal antibody is used that is directed against 4-amino-7-chloroquinoline conjugated to keyhole limpet hemocyanin by the glutaraldehyde method. Besides CLQ, this antibody also recognizes with good affinity the 4-aminoquinoline homologs, hydroxychloroquine and amodiaquine. No extraction step or sample preparation is required, and the method can detect as little as 10 micrograms/L, the lower concentration in plasma of humans who are taking CLQ as a preventive measure. The between-assay CV is less than or equal to 10%, the within-assay CV less than or equal to 3%. Results correlate with those by liquid chromatography (r2 = 0.96). The speed and simplicity of the RIA method make it useful in evaluating the CLQ concentrations in acutely toxic patients.
我们描述了一种基于3H的放射免疫分析法，用于检测抗疟药物氯喹（CLQ），这是最常用的抗疟药物。在该分析中，使用了一种单克隆抗体，该抗体通过戊二醛法针对与钥孔血蓝蛋白偶联的4-氨基-7-氯喹啉。除了氯喹，该抗体还能以良好的亲和力识别4-氨基喹啉同系物、羟氯喹和阿莫地喹。该方法无需萃取步骤或样品制备，能够检测低至10微克/升的浓度，这是服用氯喹作为预防措施的人体内血浆中的较低浓度。批间变异系数小于或等于10%，批内变异系数小于或等于3%。结果与液相色谱法的结果相关（r2 = 0.96）。放射免疫分析方法的速度和简便性使其在评估急性中毒患者的氯喹浓度时很有用。
