Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China.
Nutrients. 2023 Apr 25;15(9):2074. doi: 10.3390/nu15092074.
Hypertension is closely related to metabolic dysregulation, which is associated with microbial dysbiosis and altered host-microbiota interactions. However, plasma metabolite profiles and their relationships to oral/gut microbiota in hypertension have not been evaluated in depth. Plasma, saliva, subgingival plaques, and feces were collected from 52 hypertensive participants and 24 healthy controls in a cross-sectional cohort. Untargeted metabolomic profiling of plasma was performed using high-performance liquid chromatography-mass spectrometry. Microbial profiling of oral and gut samples was determined via 16S rRNA and metagenomic sequencing. Correlations between metabolites and clinic parameters/microbiota were identified using Spearman's correlation analysis. Metabolomic evaluation showed distinct clusters of metabolites in plasma between hypertensive participants and control participants. Hypertensive participants had six significantly increased and thirty-seven significantly decreased plasma metabolites compared to controls. The plasma metabolic similarity significantly correlated with the community similarity of microbiota. Both oral and gut microbial community composition had significant correlations with metabolites such as Sphingosine 1-phosphate, a molecule involved in the regulation of blood pressure. Plasma metabolites had a larger number of significant correlations with bacterial genera than fungal genera. The shared oral/gut bacterial genera had more correlations with metabolites than unique genera but shared fungal genera and metabolites did not show clear clusters. The hypertension group had fewer correlations between plasma metabolites and bacteria/fungi than controls at species level. The integrative analysis of plasma metabolome and oral/gut microbiome identified unreported alterations of plasma metabolites in hypertension and revealed correlations between altered metabolites and oral/gut microbiota. These observations suggested metabolites and microbiota may become valuable targets for therapeutic and preventive interventions of hypertension.
高血压与代谢失调密切相关，代谢失调与微生物失调和宿主-微生物相互作用改变有关。然而，高血压患者的血浆代谢物谱及其与口腔/肠道微生物群的关系尚未得到深入评估。在一项横断面队列研究中，从 52 名高血压患者和 24 名健康对照者中收集了血浆、唾液、龈下斑块和粪便。使用高效液相色谱-质谱法对血浆进行非靶向代谢组学分析。通过 16S rRNA 和宏基因组测序确定口腔和肠道样本的微生物分析。使用 Spearman 相关分析确定代谢物与临床参数/微生物群之间的相关性。代谢组学评价显示高血压患者和对照组患者血浆中存在明显不同的代谢物簇。与对照组相比，高血压患者有六种显著增加的和三十七种显著减少的血浆代谢物。血浆代谢相似性与微生物群的群落相似性显著相关。口腔和肠道微生物群落组成与代谢物如 Sphingosine 1-phosphate 均有显著相关性，Sphingosine 1-phosphate 是一种参与血压调节的分子。与真菌属相比，血浆代谢物与细菌属的显著相关性更多。共享的口腔/肠道细菌属与代谢物的相关性多于独特的细菌属，但共享的真菌属和代谢物没有显示出明显的聚类。与对照组相比，高血压组在种水平上血浆代谢物与细菌/真菌之间的相关性较少。血浆代谢组和口腔/肠道微生物组的综合分析鉴定了高血压患者血浆代谢物的未报道变化，并揭示了改变的代谢物与口腔/肠道微生物群之间的相关性。这些观察结果表明，代谢物和微生物群可能成为治疗和预防高血压的有价值的靶点。
