Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands.
Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge and NIHR Biomedical Research Centre, Cambridge, UK; Department of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK.
STAR Protoc. 2023 Sep 15;4(3):102431. doi: 10.1016/j.xpro.2023.102431. Epub 2023 Jul 9.
Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases. For complete details on the use and execution of this protocol, please refer to Roos et al. (2022)..
胆管对于胆汁运输至关重要，由复杂的分支管状网络组成。源自人类患者的胆管细胞会发展成囊性而非分支状的管腔形态。在这里，我们提出了一种在胆管细胞和胆管癌类器官中建立分支形态发生的方案。我们描述了启动、维持和扩展肝内胆管细胞类器官分支形态的步骤。该方案可用于研究器官特异性和非间质依赖性分支形态发生，并为研究胆汁功能和疾病提供了一个改进的模型。有关该方案的使用和执行的完整详细信息，请参阅 Roos 等人。(2022)。
