人诱导多能干细胞衍生的输尿管芽类器官的生成和扩增方案。
Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids.
机构信息
Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Rege Nephro Co., Ltd., Med-Pharm Collaboration Building, Kyoto University, 46-29 Yoshidashimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
出版信息
STAR Protoc. 2022 Jun 17;3(3):101484. doi: 10.1016/j.xpro.2022.101484. eCollection 2022 Sep 16.
The ureteric bud (UB) is a kidney precursor tissue that repeats branching morphogenesis and gives rise to the collecting ducts (CDs) and lower urinary tract. Here, we describe protocols to generate iUB organoids from human iPSCs; iUB organoids repeat branching morphogenesis. We describe how to expand iUB-organoid-derived tip colonies and how to induce CD progenitors from iUB organoids. These organoids can be used to study CD development and potentially as a model of kidney and urinary tract diseases. For complete details on the use and execution of this protocol, please refer to Mae et al. (2020).
输尿管芽（UB）是一种肾脏前体组织，它重复分支形态发生并产生集合管（CDs）和下尿路。在这里，我们描述了从人诱导多能干细胞（iPSCs）中生成 iUB 类器官的方案；iUB 类器官重复分支形态发生。我们描述了如何扩展 iUB-类器官衍生的尖端集落，以及如何从 iUB 类器官中诱导 CD 前体细胞。这些类器官可用于研究 CD 的发育，并可能作为肾脏和泌尿道疾病的模型。有关该方案使用和执行的完整详细信息，请参见 Mae 等人。（2020 年）。
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